几类小麦雄性不育系育性敏感时期可溶性蛋白质和ATP酶活性的分析  

Analysis on Soluble Proteins and ATPase Activities of Male Sterile Wheat Lines during Fertility Sensitive Period

作　　者：王爱方[1] 马翎健[1] 李艳飞[1] 武晗[1] 靳凤[1] 何蓓如[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《麦类作物学报》2010年第3期539-543,共5页Journal of Triticeae Crops

基　　金：西北农林科技大学学术骨干专项基金;唐仲英育种基金

摘　　要：为了研究K型和YS型小麦雄性不育系雄性不育的生化机制,用Native-PAGE凝胶电泳技术对稀盐缓冲液提取的不育系及其保持系育性敏感期旗叶和小穗中的可溶性蛋白进行了分析,并对不育系和保持系小穗质膜和液泡膜H+-ATP酶活性进行了比较。结果显示,1B/1R类K型不育系3314A和非1B/1R类K型不育系732A的同型保持系732B的旗叶在二核期和三核期均出现一分子量大小相同的特异性条带。对小穗的电泳结果显示,1B/1R类K型不育系3314A和非1B/1R类K型不育系732A单核期与其同期相应保持系相比,表达了一些特异性的蛋白,而YS型光温敏不育系A3017二核期和三核期比单核期多出现了特异蛋白条带。这些小穗中表达的特异蛋白可能与育性相关。另外,通过对质膜和液泡膜H+-ATP酶活性测定表明,不育系小穗单核期ATP酶活性较高,尤其是不育系液泡膜H+-ATP酶活性在单核期均明显偏高。To study the biochemical mechanism of male sterility in K-type and YS-type male sterile wheat lines,soluble proteins were extracted from flags and spikes of male sterile wheat lines and their maintainers at uninucleate,binucleate and trinucleate stages and analysed by Native-PAGE,and the ATPase activity in plasma membrane and tonoplast between male sterile wheat lines and their maintainers were compared.The results of Native-PAGE displayed that special proteins bands were observed in the flag-leaves of 1B/1...

关 键 词：小麦 雄性不育系 NATIVE-PAGE 蛋白质 ATP酶 

分 类 号：S512.1[农业科学—作物学]