AFLP Analysis of Cytoplasmic Male-sterile Line and Its Maintainer Line of Pepper  

辣椒细胞质雄性不育系及其保持系的AFLP分析(英文)

作　　者：罗向东[1,2] 戴亮芳[1,2] 陈劲枫[2] 王述彬[3] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]江西师范大学生命科学学院,江西南昌330022 [3]江苏省农业科学院蔬菜研究所,江苏南京210014

出　　处：《Agricultural Science & Technology》2010年第2期69-71,共3页农业科学与技术（英文版）

基　　金：Supported by National 863 Program in the 11th Five-year Planning Period(2006AA100108-3-2);National Natural Science Foundation(30860120;30900781);Science and Technology Project from Jiangxi Provincial Office of Education(GJJ08159);Young Growth Fund of Jiangxi Normol University(2398)~~

摘　　要：[Objective] The aim of this study was to analyze the cytoplasmic male sterile line 21A and its maintainer line 21B of peppers by AFLP,and lay the foundation for further studies on molecular mechanism of the cytoplasmic male sterility in peppers.[Method] Cytoplasmic male sterility(CMS)line 21A and its maintainer line 21B were analyzed by AFLP to obtain the specific amplified fragments of cytoplasmic male sterile line 21A,while the specific amplified fragments were recovered or sequenced,and analyzed by BLAST...[目的]对辣椒细胞质雄性不育系及其保持系进行AFLP分析,为进一步深入研究辣椒细胞质雄性不育的分子机制奠定基础。[方法]采用AFLP技术对辣椒细胞质雄性不育系21A及其保持系21B进行分析,获得不育系21A特异扩增片段。将特异扩增片段回收测序并用BLASTn和BLASTX程序在NCBI数据库中进行同源性检索和相似性比对。[结果]获得了不育系21A特异性扩增片段AA/CAG169,该片段中部分序列(位于122~158bp,AGAATTGGTA CGCAGTCTATGATGAGTCCTGAGTAAC,共37bp)是Penaeus monodon性别及生长性状相关的AFLP标记序列(AFE549M27.1)中串联重复序列;BLASTX和TBLASTX分析结果表明,该特异性片段的翻译产物与马铃薯的假拟gag-pol蛋白、假拟逆转座子蛋白、假拟逆转座子gag蛋白具有较高的相似性。[结论]特异性片段AA/CAG169的功能可能与逆转座子有关,表明其可能与育性密切相关。

关 键 词：PEPPER cytoplasmic male-steriltiy（CMS） AFLP analysis 

分 类 号：S641.3[农业科学—蔬菜学]