Studies of Glycine Betaine on Physiology of Two Varieties of Pumpkin Seedlings under NaCl Stress  

外源甜菜碱对盐胁迫下2种南瓜幼苗生长的影响(英文)

作　　者：李卫欣[1] 姚太梅[1] 王鹏[1] 陈贵林[2] 霍书新[1] 

机构地区：[1]河北北方学院园艺系,河北张家口075000 [2]内蒙古大学生命科学学院,内蒙古呼和浩特010021

出　　处：《Agricultural Science & Technology》2010年第2期106-108,共3页农业科学与技术（英文版）

基　　金：Supported by the National Natural Science Foundation of China(30660015);Key Laboratory of Vegetable Genetics and Physiology of Ministry of Agriculture of the People s Republic of China(2003-2005)~~

摘　　要：[Objective] The research aimed to discuss the relationship between Glycine betaine and the salt-tolerance mechanism of different pumpkins and confirm the mechanism of betaine in salt-tolerance physiology.[Method] Taking the seedlings of C.ficifolia and Qingli pumpkin as test materials,the effects of glycine betaine on the cell membrane permeability,MDA and root activity of two varieties of pumpkin seedlings under 300 mmol/L NaCl stress were studied.[Result] At suitable concentrations,glycine betaine could d...[目的]探讨甜菜碱与不同南瓜耐盐性机制之间的关系,明确甜菜碱在耐盐生理中的作用机理。[方法]以黑籽南瓜和青栗南瓜幼苗为试验材料,研究外源甜菜碱(GB)对浓度300mmol/LNaCl胁迫下2种南瓜幼苗细胞膜透性、MDA和根系活力的影响。渗透率以相对电解质渗透率表示;硫代巴比妥酸比色法测定MDA含量;根系活力采用氯化三苯基四氮唑(TTC)还原法测定;叶绿素含量采用无水乙醇和丙酮(1∶1)混合液提取法。[结果]在合适的浓度下,外源甜菜碱能降低盐胁迫下2种南瓜幼苗叶片质膜透性,抑制膜质过氧化产物MDA的积累,提高植物的根系活力。[结论]甜菜碱处理盐胁迫下的南瓜幼苗能够降低盐胁迫对细胞膜的伤害,缓解盐害,提高南瓜幼苗的耐盐性。

关 键 词：Salt stress Glycine Betaine Pumpkin seedlings 

分 类 号：S642.1[农业科学—蔬菜学]