DNase-I纯化结合碱性裂解法提取混合斑精子DNA  

The extraction of sperm DNA from mixed stain using DNase-I purification combined with alkaline lysis method

作　　者：袁自闯[1,2] 金洪年[2] 赖跃[3] 王慧君[1] 陈红英[2] 

机构地区：[1]南方医科大学法医学系,广东广州510515 [2]广东省公安厅刑事技术中心,广东广州510050 [3]深圳市公安局刑科所,广东深圳510840

出　　处：《中国法医学杂志》2010年第1期10-12,共3页Chinese Journal of Forensic Medicine

摘　　要：目的研究脱氧核糖核酸酶I(DNase-I)纯化结合碱性裂解法提取混合斑精子DNA的方法在法医学中的应用。方法收集79份性犯罪案件混合斑检材,分别用DNase-I纯化结合碱性裂解法和差异裂解法提取精子DNA,采用STR荧光标记复合扩增体系进行16个STR基因座分型,并比较检验结果。结果应用DNase-I纯化结合碱性裂解法提取精子DNA,64例检材分型成功;应用差异裂解法提取精子DNA,57例检材分型成功;两种方法比较结果存在显著性差异(P=0.039),DNase-1纯化结合碱性裂解法提取精子DNA的STR分型成功率更高,成本低廉。结论DNase-I纯化结合碱性裂解法提取混合斑精子DNA可提高检验成功率,操作简便,快速,易于自动化,适于法医学个体识别鉴定。Objective To establish a method of sperm DNA extraction in mixed stain by using DNase-I purification combined with alkaline lysis method in forensic science.Methods 79 mixed stain samples of criminal cases were collected.Sperm DNA was extracted using the purification of DNase-I binding alkaline lysis method.16 STR loci were genotyped with fluorescent multiplex amplification system.The typing results were compared with that of extracted using two-step differential extraction procedure.Results Of all 79 mixed...

关 键 词：法医物证学 脱氧核糖核酸酶I 碱性裂解法 混合斑 精子DNA 

分 类 号：D919.1[医药卫生—法医学]