人血浆中法莫替丁的测定及其药物动力学的研究  

Determination of Famotidine in human plasma by SPE-LC/MS/MS

作　　者：桂静[1] 杜青青[1] 王凌[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2010年第2期147-150,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的快速测定法莫替丁在人血浆中的浓度,并研究其药动学。方法12名健康志愿者单剂量空腹po法莫替丁片40mg,于给药后不同时间收集血浆样品,固相萃取,以阿莫西林为内标,采用液相色谱-质谱联用法测定法莫替丁在血浆中的浓度,并计算药动学参数。结果在优化条件下,法莫替丁的线性范围是0.3～400ng·mL-1(r=0.9993),最低定量限为0.3ng·mL-1,RSD=9.14%。法莫替丁片在所选受试者体内的动力学过程符合一室模型。Tmax=2.63±0.36h;Cmax=150.52±6.09ng·mL-1;AUC0-15=764.54±87.56μg·h·L-1;t1/2=2.77±0.32h。结论所建方法灵敏度高、准确度好、专属性强,分析速度快,适用于法莫替丁血药浓度的测定及其药动学的研究。OBJECTIVE To establish a sensitive and rapid liquid chromatography-tandem mass spectrometry method for determining the concentration of famotidine in human plasma,and apply it to the pharmacokinetic study of famotidine tablet.METHODS The human plasma from 12 healthy subjects were collected at various time points after administration with single oral dose of 40 mg famotidine tablet under fasted conditions.Plasma samples were pretreated by solid-phase extraction columns and the concentration of famotidine in ...

关 键 词：法莫替丁 固相萃取 液相色谱-质谱-质谱联用法 血药浓度 

分 类 号：R96[医药卫生—药理学]