HPCE测定保健品及中药中的多种黄酮类化合物  

Determination of flavonoids in health care products and Chinese patent medicine by HPCE

作　　者：卢佳[1] 孙成均[2] 叶利明[1] 钱广生[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川大学华西公共卫生学院,四川成都610041

出　　处：《华西药学杂志》2010年第2期168-170,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家"十一五"科技支撑计划课题资助(课题号:2006BAD27B02)

摘　　要：目的分离测定中药和保健品中黄芩素、异鼠李素、芦丁、儿茶素、槲皮苷、木樨草素、芹菜素等7种黄酮类化合物。方法采用高效毛细管区带电泳紫外检测法。运行缓冲液为50mmol·L-1Na2BO4·10H2O-50mmol·L-1H3BO3(pH9.5),分离电压20kV,温度20℃,检测波长245nm。结果黄芩素、异鼠李素、芦丁、儿茶素、槲皮苷、木樨草素、芹菜素分别在2.79～33.5、12.2～244、68.5～1370、4.70～470、5.75～345、2.33～28、19.9～398μg·mL-1与各自的峰面积成良好的线性关系,r分别为0.9980、0.9940、0.9974、0.9990、0.9986、0.9996、0.9979,检出限分别为1.340、0.976、0.685、2.350、1.725、0.244、0.398μg·mL-1。结论7种物质在16min内可得到良好的分离。OBJECTIVE To develop a method for the determination of flavonoids in Chinese patent medicine and health care products.METHODS HPCE-UV was used with the running buffer of 50 mmol·L -1 Na2BO4·10H2O-50 mmol·L -1 H3BO3 (pH9.5).The applied voltage was 20 kV.The column temperature was maintained at 20 ℃.The electropherogram was detected at 245nm.RESULTS The linearities of baicalein,isorhamnetin,rutin,catechin,quercitrin,luteolin and pelargidenon were obtained over the ranges of 2.79-33.5(r = 0.9980),12.2-244(r = ...

关 键 词：黄酮类化合物 中药 保健品 高效毛细管电泳法 

分 类 号：R284[医药卫生—中药学]