贵州产菝葜类8种药用植物中薯蓣皂苷元的分析  

Analysis of diosgenin of 8 species of medicinal plants from smilax group in Guizhou province

作　　者：曲国玉[1] 任广聪[1] 董凯旋[1] 王先奎[1] 朱莉[1] 董立莎[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《华西药学杂志》2010年第2期135-137,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金项目(批准号:30960503);贵州省科学技术基金(黔科合丁字[2008]2143号)

摘　　要：目的采用TLC、RP-HPLC法分析贵州产菝葜属及肖菝葜属8种常用药用植物中的指标性成分薯蓣皂苷元。方法TLC法采用硅胶G板,以环己烷-乙酸乙酯(4∶1)为展开剂。RP-HPLC的色谱条件为依利特Hypersil ODS2色谱柱(250mm×4.6mm,5μm),流动相为甲醇-0.2%磷酸(76∶24),流速1.0mL·min-1,检测波长203nm,柱温30℃,薯蓣皂苷元理论板数不低于4×103。结果薄层鉴别中仅有短梗菝葜、黑叶菝葜及小叶菝葜含有薯蓣皂苷元。薯蓣皂苷元0.0105～2.105μg与峰面积呈良好的线性关系;平均加样回收率为100.4%(RSD=1.85%,n=9)。黑叶菝葜的薯蓣皂苷元含量最高,为0.15%,土茯苓、柔毛菝葜、托柄菝葜、短柱肖菝葜中均未检测到薯蓣皂苷元。结论所用TLC法及RP-HPLC法简单易行、专属性强、准确度高、重复性好,可作为菝葜类药材内在质量的控制方法。OBJECTIVE To establish a TLC method and RP-HPLC analysis of diosgenin as the indicator composition in 8 kinds of Guizhou Smilax (Smilax L.) and Xiao Smilax(Heterosmilax K.),the commonly used medicinal plants.METHODS TLC method,using silica gel G plate with cyclohexane-ethyl acetate(4∶1)as the agent,was carried out for qualitative analysis.Using RP-HPLC determination of eight varieties of diosgenin content,chromatographic conditions were as follows: Bartlett Hypersil ODS2 column(250 mm × 4.6 mm,5 μm);mobile ...

关 键 词：薯蓣皂苷元 菝葜类 薄层色谱法 反相高效液相色谱法 

分 类 号：R284[医药卫生—中药学]