RP-HPLC测定阿戈美拉汀原料药的含量  

Determination of Agomelatine by RP-HPLC

作　　者：何学军[1] 任真[1] 王娟[1] 刘晶[2] 杜宇[1] 乔红群[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京21009 [2]江苏省药物安全性评价中心,江苏南京210009

出　　处：《华西药学杂志》2010年第2期204-205,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用RP-HPLC法测定阿戈美拉汀原料药的含量。方法色谱柱为Plastil-C18柱(150mm×4.6mm,5μm),流动相为乙腈-水(30∶70),流速1.0mL·min-1,检测波长为230nm。结果线性回归方程为:Y=2.283×104X-1.353×104(r=0.9999),线性范围为5～80μg·mL-1,平均回收率为100.4%(n=9),RSD=0.97%。结论所建方法简便、快速、灵敏度高,可用于阿戈美拉汀原料药的含量测定。OBJECTIVE A method for determination of Agemelatine.METHODS An HPLC method was adopted with Plastil-C18 column(150 mm × 4.6 mm,5 μm).The mobil phase consisted of acetonitrile and water(30∶70)with a flow rate of 1 mL·min -1 and the detective wavelength was at 230 nm.RESULTS The calibration curve of agemelatine was linear( r = 0.9999) in the range of 5.0-80.0 μg·mL -1.The average recovery of agemelatine was 100.4% (n = 9)with RSD of 0.97% .CONCLUSION The method is accurate,reliable and simple.It can be applie...

关 键 词：阿戈美拉汀 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]