HPLC测定舒肤软膏中的蒿酮  

Determination of artemisia ketone in Shufu ointment by HPLC

作　　者：田吉[1] 何兵[1] 申丽英[2] 

机构地区：[1]泸州医学院药学院,四川泸州646000 [2]泸州医学院附属医院,四川泸州646000

出　　处：《华西药学杂志》2010年第2期213-214,共2页West China Journal of Pharmaceutical Sciences

基　　金：四川省教育厅青年基金项目(2000-B12)

摘　　要：目的采用HPLC法测定舒肤软膏中蒿酮的含量。方法色谱柱为Dikma Diamonsil C18柱(250mm×4.6mm,5μm),流动相为甲醇-水(80∶20),流速0.8mL·min-1,检测波长243nm。结果蒿酮0.2205～3.5272μg与峰面积的线性关系良好(r=0.9999),平均加样回收率为98.37%。结论所用方法灵敏、准确、快速,重复性好,可作为该制剂的定量分析方法。OBJECTIVE To establish an HPLC method for the determination of Artemisia ketone in Shufu ointment.METHODS The separation was carried out on a Dikma Diamonsil C18 column (250 mm × 4.6 mm,5 μm).Mobile phase consisting of methanol-water (80∶20) was adopted,and the detection wavelength was set at 243 nm.The flow rate was 0.8 mL·min -1.RESULTS The calibration curve showed good linearity over the range of 0.2205-3.5272 μg(r = 0.9999).The mean average recovery was 98.37% .CONCLUSION The method is sensitive,accurat...

关 键 词：舒肤软膏 蒿酮 含量测定 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]