RP-HPLC测定小鼠脑匀浆中的盐酸尼莫司汀  

Determination of Nimustine hydrochloride in mouse brain homogenate by RP-HPLC

作　　者：王雪[1] 巫孟君[1] 贾钰铭[2] 张文彬[3] 陈华黎[1] 覃瑶[1] 何勤[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]宜宾市第二人民医院,四川宜宾644000 [3]四川省肿瘤医院,四川成都610041

出　　处：《华西药学杂志》2010年第2期179-180,共2页West China Journal of Pharmaceutical Sciences

基　　金：四川大学大学生创新性实验计划国家重点项目

摘　　要：目的采用HPLC法测定小鼠脑匀浆中的盐酸尼莫司汀。方法使用Diamonsil C18柱,流动相为甲醇-水-三乙胺(20∶79.5∶0.5,磷酸调pH3),流速1.0mL·min-1,检测波长241nm,柱温35℃。结果盐酸尼莫司汀0.20～5.00mg·L-1与峰面积的线性关系良好(r=0.9999);平均方法回收率95.7%,平均萃取回收率101.2%,日内、日间RSD均<5%。结论该方法简便、灵敏、准确,可用于测定脑匀浆中的盐酸尼莫司汀。OBJECTIVE To establish a method for the determination of nimustine hydrochloride in mouse brain homogenate.METHODS The concentration of nimustine hydrochloride was determined using HPLC-UV with the Diamonsil C18 column.The mobile phase was methanol-water-triethylamine (20∶79.5∶0.5,adjusted to pH3 with H3PO4).The flow rate was 1.0 mL·min -1.The detection wavelength was at 241nm,and the column temperature was 35 ℃.RESULTS The linear range was 0.20-5.00 mg· L -1(r = 0.9999).The average methodological recovery ...

关 键 词：盐酸尼莫司汀 脑匀浆 高效液相色谱法 小鼠 

分 类 号：R965[医药卫生—药理学]