亚硫酸氢钠穿心莲内酯对小鼠和家兔的肾毒性作用  

Toxic actions of andrographolide sodium bisulfite on kidneys of mice and rabbits

作　　者：陆红[1] 张信岳[2] 周彦荃[1] 金少圣[1] 

机构地区：[1]浙江中医药大学药学院药理教研室,浙江杭州310053 [2]浙江省医学科学院药物研究所药理研究室,浙江杭州310013

出　　处：《中国药理学与毒理学杂志》2010年第3期223-227,共5页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的观察亚硫酸氢钠穿心莲内酯(ASB)的肾毒性作用。方法 30只ICR小鼠随机分为正常对照组、ASB150和1000mg.kg-1组,ASB组小鼠尾静脉iv给予ASB,每天1次,连续7d,正常对照组iv给予等体积生理盐水,检测体重、肾脏系数、血常规、血清尿素氮和肌酐浓度,观察肾脏组织病理变化等指标。18只新西兰家兔,随机分为对照组、ASB125和500mg.kg-1组,麻醉后单次经耳缘静脉iv给予ASB,导尿管法收集尿液并测定尿量及尿常规指标。结果与正常对照组比较,ASB1000mg.kg-1可使小鼠血清肌酐和尿素氮浓度分别从124±19和18±3升高至197±35和(35±10)mmol.L-1(P<0.05),并使外周血白细胞从(10.5±2.0)×109L-1升高至(13.7±3.4)×109L-1,血小板从(666±122)×109L-1降低至(451±207)×109L-1,淋巴细胞比例从(0.83±0.03)降低至(0.68±0.11)(P<0.05),7/10小鼠肾脏出现间质性肾炎,伴轻度纤维化,部分肾小管有蛋白,近曲小管浊肿;与正常对照组比较,ASB150mg.kg-1对小鼠上述指标无明显影响。ASB500mg.kg-1可引起家兔尿蛋白和酮体阳性检出率一过性增加,给药后120min总尿量明显增加(P<0.05);与正常对照组比较,ASB125mg.kg-1对家兔上述指标无明显影响。结论 ASB1000mg.kg-1和500mg.kg-1时分别对小鼠和家兔肾脏具有毒性作用。OBJECTIVE To observe the renal toxicity of andrographolide sodium bisulfite (ASB).METHODS Thirty ICR mice were randomly divided into normal control,ASB 150 and 1000 mg·kg-1 groups,with 10 mice in each group.Mice in ASB groups were iv given ASB 150 and 1000 mg·kg-1 by tail vein,once daily,for 7 d.The body weight,renal index,blood routine,serum urea nitrogen and creatinine were assessed,and histopathological changes in renal tissues were detected.Eighteen New Zealand rabbits were randomly divided into normal ...

关 键 词：亚硫酸氢钠穿心莲内酯 药物毒性 肾 

分 类 号：R285.5[医药卫生—中药学]