穿心莲内酯对小鼠T淋巴细胞体外活化、增殖及细胞周期的影响  

The effect of andrographolide on activation,proliferation and cell-cycle of mouse T lymphocytes in vitro

作　　者：赵晓慧[1] 曾耀英[1] 慕静静[1] 宋兵[1] 

机构地区：[1]暨南大学生命科学技术学院组织移植与免疫中心,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2009年第4期373-378,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金："973"国家重大基础研究项目(2006CB504200;2004CB720100);广东省基金项目(2006B36030016);广州市科技局科技攻关重点项目(2006Z-E0091);生物化学与分子生物学广东省重点学科资助项目

摘　　要：目的:研究穿心莲内酯对小鼠T淋巴细胞体外活化、增殖及细胞周期的影响,并对其免疫调节作用进行初步探讨。方法:分离小鼠淋巴结细胞,以双色荧光抗体染色技术结合流式细胞术检测在刀豆蛋白A(Con A)的刺激下,穿心莲内酯对T淋巴细胞早期活化抗原CD69表达的影响;用羧基荧光素乙酰乙酸(CFDA-SE)染色分析穿心莲内酯对T淋巴细胞增殖的影响;用碘化丙锭染色分析细胞周期的分布。结果:终浓度为10、20、30、40μmol/L的穿心莲内酯对Con A刺激的早期活化抗原CD69的表达具有明显的抑制作用(P<0.01);对Con A刺激的T淋巴细胞增殖具有明显的抑制作用(P<0.01);并明显阻止T淋巴细胞进入S期,且呈剂量依赖性。结论:穿心莲内酯对小鼠T淋巴细胞的体外活化及增殖均具有明显的抑制作用,并抑制T淋巴细胞进入分裂周期。Aim:To investigate the effects of andrographolide on activation,proliferation and cell-cycle,to elucidate the mechanism of the immunosuppressive effect of andrographolide. Methods: Cell suspensions were prepared from mouse lymph nodes.T lymphocytes were treated with different concentrations of andrographolide,and stimulated with polyclonal activators Con A.Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69.After the staining with CFDA-SE,T lymphocytes were stimulated with polyclonal activators Con A.Flow cytometry(FCM) was used to detect the proliferation of T lymphocytes.The cell-cycle distribution was analyzed by propidium iodide staining.Results: In a dose-dependent manner,andrographolide(10,20,30,40 μmol/L) can significantly inhibit T lymphocytes activation index(P<0.01) stimulated by Con A,and inhibit proliferation index(P<0.01) stimulated by Con A,as well as the progression of T lymphocytes in S phase.Conclusion:Andrographolide can effectively inhibit activation,proliferation and cell-cycle of mouse T lymphocytes,so we can conclude that andrographolide is a potential effective immuno-inhibitory agent.

关 键 词：穿心莲内酯 活化 增殖 细胞周期 流式细胞术 

分 类 号：R392.1[医药卫生—免疫学]