Molecular Cloning of a Cytosolic G6PDH Gene from Populus Suaveolens and Its Expression to Improve the Cold Resistance of Tobacco Plants  

作　　者：LIN Yuan-zhen1,2,GUO Hai3,ZHANG Zhi-yi2,LIN Shan-zhi2 (1. College of Forestry,South China Agricultural University,Guangzhou Guangdong 510642,China 2. Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants,MOE,Beijing Forestry University,Beijing 100083,China 3. Plant Materials Center for Soil and Water Conservation,Ministry of Water Resources,Beijing100038,China) 

出　　处：《冰川冻土》2009年第6期1178-1185,共8页Journal of Glaciology and Geocryology

基　　金：supported by the National Natural Science Foundation of China (No .30271093)

摘　　要：Glucose-6-phosphate dehydrogenase (G6PDH,EC 1.1.1.49) is the first and main regulated enzyme of oxidative pentose phosphate pathway (OPPP),catalyzing the conversion of glucose-6-phosphate to 6-phospho-gluconolactone and playing important roles in the growth and development of plants. It is preciously reported that the enhancement of freezing resistance of Populus suaveolenscuttings is clear related to the distinct increase in cytosolic G6PDH activity. Here,a 1697 bp cDNA fragment (PsG6PDH) is amplified by RT-PCR from cold-induced total RNA of the freezing-tolerant P. suaveolens. A sequence analysis showed that PsG6PDH coding region had 1 530 bp and encoded 510 predicted amino acid residues. Genomic Southern analysis revealed that the isoform is encoded by a few copies of the gene in the poplar genome. The cloned gene PsG6PDHis cloned into binary vector pBI121 and used to transform tobacco. PCR and Southern blotting results verified integration of this gene into the genome of tobacco. Moreover,cold treatment experiments and membrane defense enzymeactivity analysis confirmed that overexpression of the PsG6PDHgene could enhance the tolerance to cold or frigid stresses in transgenic plants.Glucose-6-phosphate dehydrogenase (G6PDH,EC 1.1.1.49) is the first and main regulated enzyme of oxidative pentose phosphate pathway (OPPP),catalyzing the conversion of glucose-6-phosphate to 6-phospho-gluconolactone and playing important roles in the growth and development of plants. It is preciously reported that the enhancement of freezing resistance of Populus suaveolenscuttings is clear related to the distinct increase in cytosolic G6PDH activity. Here,a 1697 bp cDNA fragment (PsG6PDH) is amplified by R...

关 键 词：Populus suaveolens glucose-6-phosphate dehydrogenase cold resistance gene engineering 

分 类 号：Q943.2[生物学—植物学]