束缚+温水(36±1)℃应激对大鼠延髓及下丘脑Fos蛋白表达的影响  

The Effect of Restraint Warm Water-immersion Stress(36±1)℃ on the Fos Expression in Medulla and Hypothalamus of Rat

作　　者：李慧[1] 纪丽珍[1] 赵玉琳[1] 艾洪滨[1] 

机构地区：[1]山东师范大学生命科学学院,山东省动物抗性生物学重点实验室,济南250014

出　　处：《生物医学工程研究》2009年第4期259-262,共4页Journal Of Biomedical Engineering Research

基　　金：国家自然科学基金资助项目(30770277);国家自然科学基金资助项目(30970354)

摘　　要：为了探讨束缚+温水(36±1)℃应激是否能引起大鼠胃粘膜损伤、是否对延髓和下丘脑Fos蛋白表达有影响,本研究将雄性Wistar大鼠随机分两组:实验组,束缚+温水(36±1)℃应激1 h;对照组,室温下单纯束缚应激1 h。应激后处死,测直肠温度;取胃,观察胃粘膜损伤程度;取脑,应用免疫组化染色方法观察两组动物延髓、下丘脑神经元的Fos蛋白表达。结果显示:两组动物胃粘膜损伤程度均较轻或基本无胃粘膜损伤,延髓和下丘脑各核团Fos蛋白表达均无显著性差异,直肠温度也无显著性差异。这些结果提示,由于束缚+温水(36±1)℃应激不改变动物的体温,因而不引起延髓和下丘脑控制胃机能的核团神经元活动加强,从而也不诱发急性胃粘膜损伤。To investigate the effect of restraint warm water-immersion stress on the Fos expression in medulla and hypothalamus of rat,male Wistar rats weighting 150～200 g were randomly divided into two groups: experimental group,control group.The experimental group was restrained and immersed in warm water(36±1)℃;the control group was only restrained under the room temperature.One hour after stress,the rats were sacrificed.The rectum temperature was measured,the gastric mucosal ulcer index was determined according to...

关 键 词：束缚+冷水应激 束缚+温水应激 FOS蛋白 延髓 下丘脑 

分 类 号：R338[医药卫生—人体生理学]