抗朊蛋白单克隆抗体的免疫学特性研究  

Immunological characteristics of Anti-PrP McAbs

作　　者：吴晓东 张永强[2] 刘雨田[2] 邹艳丽[2] 刘珊[2] 李林[2] 王志亮[2] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州225009 [2]中国动物卫生与流行病学中心-国家外来动物疫病诊断中心-国家牛海绵状脑病参考实验室,山东青岛266032

出　　处：《扬州大学学报（农业与生命科学版）》2009年第4期6-10,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家"十一五"科技支撑计划项目(2006BAD06A13);转基因生物新品种培育科技重大专项(2009ZX08008-001B)

摘　　要：以牛、羊重组朊蛋白(PrP)、正常牛、羊的脑组织匀浆、牛海绵状脑病(BSE)及羊痒病阳性的脑组织匀浆及切片为抗原,测定9株抗朊蛋白单克隆抗体的ELISA、免疫印迹及免疫组化等免疫学反应特性。结果表明:5株单抗可用于BSE及羊痒病的ELISA、免疫印迹检测;3株单抗可用于BSE及羊痒病的免疫组化检测;1株单抗在免疫印迹试验中只与羊痒病PrPSc的核心片段反应,结合该株单抗所识别的抗原表位PrP第78~93氨基酸(aa),证实BSE与羊痒病PrPSc的蛋白酶K消化位点存在差异。In recent years,a series of anti-PrP monoclonal antibodies(McAbs) had been developed in our lab.In this research,9 McAbs immuno-reactivities with PrP were tested in ELISA,Western blotting and immunohistochemistry(IHC) assay.The results indicated that 5 McAbs could be used for the detection of BSE and Scrapie in Western blotting and ELISA,and 3 McAbs could be used for the detection of BSE and Scrapie in IHC assay.1 McAb only recognized the core part of PrPSc from sheep in Western blotting,and its epitope was...

关 键 词：传染性海绵状脑病 朊蛋白 单克隆抗体 免疫学特性 

分 类 号：S852.43[农业科学—基础兽医学]