间苯二酚与牛血清白蛋白的作用机理  

Interaction of m-dihydroxybenzene with bovine serum albumin

作　　者：梁彦秋[1] 孙小寒[1] 冯婧微[1] 王丽多[1] 臧树良[2] 

机构地区：[1]沈阳化工学院环境与生物系,沈阳110142 [2]辽宁石油化工大学化学系,抚顺113001

出　　处：《分析试验室》2010年第6期81-84,共4页Chinese Journal of Analysis Laboratory

基　　金：辽宁省教育厅基金(20060687)项目资助

摘　　要：采用荧光和紫外光谱法研究了间苯二酚与牛血清白蛋白(BSA)的相互作用。间苯二酚使BSA的构象发生改变,α-螺旋含量减小。同步荧光光谱发现间苯二酚使BSA色氨酸残基的疏水性降低,酪氨酸残基的疏水性增强。荧光光谱表明猝灭机理为静态猝灭,计算了复合物的结合常数,通过热力学参数得出间苯二酚与BSA之间的作用力主要是静电作用力。The interaction between m-dihydroxybenzene and bovine serum albumin (BSA) was investigated by fluorescence and absorption spectroscopy.m-dihydroxybenzene had strong impact on protein conformation with decrease of α-helical content of the protein.The hydrophobicity around tryptophanyl residues was weakened and the hydrophobicity around tyrosyl residues was intensified in the presence of m-dihydroxybenzene by means of synchronous fluorescence spectroscopy.The result of fluorescence spectra showed that the que...

关 键 词：间苯二酚 牛血清白蛋白 荧光光谱 紫外光谱 

分 类 号：R96[医药卫生—药理学]