铝敏化荧光法测定甲磺酸加替沙星  

Study on determination of gatifloxacin mesilate by Al(Ⅲ) sensitized fluorescence spectrometry

作　　者：孙雪花[1] 齐广才[1] 高楼军[1] 师亚莉[1] 刘维江[1] 

机构地区：[1]延安大学化学与化工学院,延安716000

出　　处：《分析试验室》2010年第7期34-36,共3页Chinese Journal of Analysis Laboratory

基　　金：陕西省教育厅专项科研基金(08JK489);陕西省延安大学校内基金(YDK2008-27)项目资助

摘　　要：研究了甲磺酸加替沙星的铝敏化荧光性质。实验发现,在pH6.00的邻苯二甲酸氢钾-NaOH缓冲体系中,溶液中的铝(Ⅲ)能与甲磺酸加替沙星反应生成稳定的络合物从而增强甲磺酸加替沙星的内源性荧光,使其荧光强度显著增强。据此建立了铝(Ⅲ)-甲磺酸加替沙星体系荧光增强法测定微量甲磺酸加替沙星的新方法。该体系在λex=365nm,λem=470nm时,甲磺酸加替沙星的浓度在1.28×10-8～3.15×10-6mol/L范围内与荧光强度呈良好的线性关系,方法检出限为8.6×10-9mol/L。相对标准偏差为1.3%(c=2.0×10-7mol/L,n=5)。此方法已成功用于药物制剂中甲磺酸加替沙星含量的测定。A fluorescence method for the determination of gatifloxacin mesilate based on the system Al(Ⅲ)-gatifloxacin mesilate was developed. In C6H4CO2HCO2K-NaOH (pH 6.00) medium,Al(Ⅲ) reacted with gatifloxacin mesilate to form a complex,which could enhance the fluorescence intensity of gatifloxacin mesilate greatly. The experimental conditions that influence the fluorescence intensity were investigated and optimized. The excitation and emission wavelengths were 365 and 470 nm,respectively. The linear relation-ship ...

关 键 词：甲磺酸加替沙星 铝 荧光光度法 

分 类 号：R96[医药卫生—药理学]