高效液相色谱法测定生物样品中多种蝶呤类化合物  

Determination of four pterins in human urine by HPLC with fluorescence detection

作　　者：谭婷[1] 万益群[2,1] 

机构地区：[1]南昌大学分析测试中心,南昌330047 [2]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《分析试验室》2010年第8期33-36,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(NO:20765002)项目资助

摘　　要：建立了一种同时分离测定人体尿液中4种蝶呤类化合物(新蝶呤、异黄蝶呤、蝶呤和生物蝶呤)的高效液相色谱法。采用SHIMADZU Shim-pack:Vp-ODS(250 mm×4.6 mm×5μm)色谱柱结合荧光检测器,在流动相为甲醇-水(10+90),流速1.0mL/min,荧光检测波长Ex390 nm,Em450 nm,柱温为室温的色谱条件下,4种蝶呤类化合物分离效果良好。尿液经0.45μm的一次性滤膜过滤,取10μL滤液直接进样测定。结果表明,各组分的线性范围为:新蝶呤0.05～1.20μg/mL,异黄蝶呤0.05～0.80μg/mL,蝶呤0.05～1.00μg/mL,生物蝶呤0.05～1.00μg/mL。4种组分的检测限均为0.01μg/mL。该方法可应用于临床癌症病人和健康人尿样中4种蝶呤类化合物的检测。A simple and fast method for the determination of four pterins(Neopterin,Isoxanthopterin,Pterin and Biopterin) in human urine by High-Performance Liquid Chromatography-fluorescence detection has been established.After being filtrated,urine samples were analyzed directly.They were separated on a Lichrospher C18 column by using methanol-water(10+90) as mobile phase at the speed of 1.0 mL/min and detected at the excitation and emission wavelengths of 390 and 450 nm.The proposed method has successfully eliminat...

关 键 词：蝶呤类化合物 高效液相色谱 荧光检测 人体尿液 

分 类 号：Q503[生物学—生物化学]