微流控芯片测定丁溴东莨菪碱注射液中丁溴东莨菪碱  

Determination of scopolamine butylbromide in scopolamine butylbromide injection by microfluidic chip

作　　者：李永冲[1] 蔡自由[1] 王充[1] 陈缵光[2] 

机构地区：[1]广东食品药品职业学院,广州510520 [2]中山大学药学院,广州510089

出　　处：《分析试验室》2010年第8期120-122,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(20575080;20727006)资助

摘　　要：建立了微流控芯片非接触电导检测法测定丁溴东莨菪碱注射液中丁溴东莨菪碱含量的方法。优化选择3 mmol/LHAc+2 mmol/LNaAc(pH4.5)为缓冲溶液,1.0 mmol/Lβ-CD为添加剂,分离电压为2.00 kV,进样时间为10 s,丁溴东莨菪碱在2 min内可得到较好分离,线性范围为20～250μg/mL(r=0.998),检出限为6.0μg/mL(S/N=3),RSD为2.1%,加标回收率为97.0%～99.6%。A new method was established for the determination of scopolamine butylbromide in scopolamine butylbromide injection by microfluidic chip with contactless conductivity detection.The optimal conditions,such as the variety and concentration of buffer solution and additive,separation voltage and injection time,were investigated.The results showed that scopolamine butylbromide was separated and detected within 2 min using 3 mmol/L HAc + 2 mmol/L NaAc(pH 4.5) as buffer solution,with 1% β-CD at the separation vol...

关 键 词：微流控芯片 丁溴东莨菪碱 非接触电导检测 测定 

分 类 号：TQ460.72[医药卫生—药物分析学]