香蕉皮中3-甲基-7,8-二羟基异色满酮-4的含量测定  

Determination of 3-methyl-7,8-dihydroxy-isochroman-4-one in the peel of Musa sapientum

作　　者：钱海[1] 黄文龙[1] 饶光玲[1] 葛亮[1] 吴晓明[1] 

机构地区：[1]中国药科大学药学院,南京210009

出　　处：《中国药科大学学报》2009年第6期524-526,共3页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.30873144);中国药科大学引进人才项目资助(No.01211096)~~

摘　　要：目的:建立香蕉皮中3-甲基-7,8-二羟基异色满酮-4的HPLC含量测定方法。方法:色谱柱为岛津ODS-C18柱(250 mm×4.6 mm,5μm),流动相:乙腈-水-磷酸(9∶91∶0.1),检测波长283 nm,流速1 mL/min,柱温35℃。结果:不同批次香蕉皮原药材中3-甲基-7,8-二羟基异色满酮-4的含量为0.029 5%～0.036 7%。检测线性范围1.09～13.08μg,平均加样回收率100.0%,RSD为0.64%(n=9)。结论:该法可作为衡量香蕉皮质量的依据。Aim:To establish an HPLC method for the determination of 3-methyl-7,8-dihydroxy-isochroman-4-one in the peel of Musa sapientum.Methods:A column of Shimadzu ODS-C18 column(250 mm×4.6 mm,5 μm),and a mobile phase of acetonitrile-water-phosphoric acid(9∶91∶0.1) were adopted.A detect wavelength of 283 nm,a flow rate of 1 mL/min,and a column temperature of 35 °C were set.Results:The content of 3-methyl-7,8-dihydroxy-isochroman-4-one in the peel of Musa sapientum was 0.029 5%-0.036 7%.Calibration curve was linear ...

关 键 词：香蕉皮 3-甲基-7 8-二羟基异色满酮-4 含量测定 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]