参薯种质遗传多样性的ISSR分析  

Genetic diversity of Dioscorea alata based on ISSR analysis

作　　者：吴志刚[1] 冷春鸿[1] 陶正明[1] 魏余煌[2] 姜程曦[1] 

机构地区：[1]浙江省亚热带作物研究所,浙江温州325005 [2]瑞安市农林局,浙江瑞安325200

出　　处：《中国中药杂志》2009年第23期3017-3020,共4页China Journal of Chinese Materia Medica

基　　金：温州市科技计划项目(N20080008);瑞安市科技计划项目(20082037)

摘　　要：目的:研究参薯亲缘关系和遗传多样性。方法:收集20个样品并对其原植物进行鉴定,样品进行ISSR分析,NTSYS-pc计算遗传相似系数,UPGMA方法聚类。结果:20个样品被鉴定为参薯、褐苞薯蓣、山薯。参薯与褐苞薯蓣、山薯存在明细的遗传差异,且其种内差异十分显著,遗传相似性系数为0.672 9~0.990 7。UPGMA聚类显示参薯种内16个样品可划分为4组,对照样品原植物表型特征,可见断面颜色及块茎支根数是评价参薯种质亲缘关系的重要依据。结论:参薯种内遗传多样性水平高,研究为山药区分提供了分子生物学依据,也为参薯新品种选育奠定了基础。This article assessed the genetic relationship and genetic diversity in Dioscorea alata.Twenty samples were examined to identify their original plants,and analyzed by ISSR markers.The results showed that 20 samples were classified into three different plants,such as D.alata,D.persimilis,and D.fordii.There was significant difference in genetic similarity coefficient between D.alata and D.persimi as well as D.fordii.There was distinct differences in D.alata,the genetic similarity coefficient was resulted from...

关 键 词：山药 参薯 山薯 褐苞薯蓣 遗传多样性 ISSR 

分 类 号：S567.239[农业科学—中草药栽培]