喜脑宁对大鼠全脑缺血的保护作用及机制研究  

Protective effect of Xinaoning freezedrying power(XNN) against global cerebral ischemia-reperfusion injury

作　　者：尹艳艳[1] 曹曦[1] 李维祖[1] 

机构地区：[1]安徽医科大学药理教研室,安徽合肥230032

出　　处：《中国中药杂志》2009年第23期3087-3091,共5页China Journal of Chinese Materia Medica

摘　　要：目的:探讨喜脑宁冻干粉(XNN)对全脑缺血的保护作用及其可能机制。方法:采用四动脉结扎法建立大鼠全脑缺血/再灌注模型,观察XNN对脑缺血再灌注过程中脑电图及翻正反射恢复时间、脑含水量、脑指数、脑组织匀浆MDA和LD含量、SOD,LDH和NOS活性、海马ET含量的影响;观察XNN对ADP诱导的血小板聚集率的影响;采用Fura-2/AM负载法,观察XNN对血小板内静息[Ca2+]i和CaCl2诱导血小板内[Ca2+]i浓度变化的影响。结果:在全脑缺血再灌注模型上,XNN可以缩短翻正反射恢复时间,促进脑电图的恢复;降低脑含水量、脑指数;提高脑组织SOD,LDH活性,降低LD,MDA含量和NOS活性;降低海马组织ET含量。XNN对ADP诱导的血小板聚集有一定的抑制作用,能降低血小板内静息期[Ca2+]i,并对CaCl2诱导的血小板内[Ca2+]i的升高有抑制作用。结论:XNN对全脑缺血具有一定的保护作用。Objective:To study the protective effects of Xinaoning freezedrying power(XNN) against global cerebral ischemia-reperfusion injury.Method:Pulsinelli four-vessel occlusion method was used to make global cerebral ischemia-reperfusion model of rats.The EEG and the reappearing time of righting reflex were recorded,and the activity of SOD,LDH,NOS and the content of MDA and LD in the brain were tested.The ET content in hippocampi was researched by radioimmunoassay method.Aggregation of platelets induced by ADP wa...

关 键 词：脑缺血 喜脑宁 脑电图 自由基 钙 

分 类 号：R285.5[医药卫生—中药学]