长梗秦艽酮通过调节Akt和ERK1/2通路抑制人肝癌BEL-7402细胞生长  

WT inhibit human hepatocellular carcinoma BEL-7402 cells growth by modulating Akt and ERK1/2 phosphorylation

作　　者：章漳[1,2] 段朝辉[1] 丁侃[2] 王峥涛[1] 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验室,上海201203 [2]中国科学院上海药物所糖生物学及糖化学实验室,上海201203

出　　处：《中国中药杂志》2009年第24期3277-3280,共4页China Journal of Chinese Materia Medica

基　　金：国家重点自然科学基金项目(30770484)

摘　　要：目的:研究长梗秦艽酮抑制人肝癌BEL-7402细胞生长与Akt和ERK1/2通路的关系。方法:采用MTT法观察BEL-7402细胞活性,Western blot蛋白质印记法检测Akt和ERK1/2磷酸化水平,流式细胞术分析细胞周期。结果:长梗秦艽酮能够抑制人肝癌BEL-7402细胞生长,诱导S期细胞周期阻滞,激活Akt和ERK1/2磷酸化,并且Akt和ERK1/2抑制剂能够拮抗长梗秦艽酮对BEL-7402细胞的生长抑制和S期阻滞诱导作用。结论:长梗秦艽酮可以通过调节Akt和ERK1/2通路诱导肿瘤细胞周期阻滞,从而抑制肿瘤细胞生长。Objective: To investigate effects of Akt and ERK1/2 signaling pathways on waltonitone(WT) induced cell growth inhibition in human hepatocellular carcinoma BEL-7402 cells.Method: Cell viability of BEL-7402 cells was examined using MTT assay.Phosphorylation of E Akt and RK1/2 were detected by Western blot analysis,while cell cycle distribution of BEL7402 cells was analyzed by flow cytometry.Result: WT could inhibit the BEL-7402 cells growth,induce the S-phase cell cycle arrest,activate Akt and ERK1/2 phospory...

关 键 词：长梗秦艽酮 BEL-7402 AKT ERK1/2 

分 类 号：R285[医药卫生—中药学]