Ribosomal DNA gene and phylogenetic relationships of Diplura and lower Hexapods  

作　　者：栾云霞 张亚平 岳巧云 庞峻峰 谢荣栋 尹文英 

机构地区：[1]Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences [2]Laboratory of Cellular and Molecular Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences

出　　处：《Science China(Life Sciences)》2003年第1期67-76,共10页中国科学（生命科学英文版）

基　　金：the National Natural Science Foundation of China (Grant No. 30130040) and Life Science Special Funds of Chinese Academy of Sciences.

摘　　要：The monophyly of Diplura and its phylogenetic relationship with other hexapods are important for understanding the phylogeny of Hexapoda. The complete 18SrRNA gene and partial 28SrRNA gene (D3-D5 region) from 2 dipluran species (Campodeidae and Japygidae), 2 proturan species, 3 collembolan species, and 1 locust species were sequenced. Combining related se-quences in GenBank, phylogenetic trees of Hexapoda were constructed by MP method using a crustacean Artemia salina as an outgroup. The results indicated that: (i) the integrated data of 18SrDNA and 28SrDNA could provide better phylogenetic information, which well supported the monophyly of Diplura; (ii) Diplura had a close phylogenetic relationship to Protura with high boot-strap support.The monophyly of Diplura and its phylogenetic relationship with other hexapods are important for understanding the phylogeny of Hexapoda. The complete 18SrRNA gene and partial 28SrRNA gene (D3-D5 region) from 2 dipluran species (Campodeidae and Japygidae), 2 proturan species, 3 collembolan species, and 1 locust species were sequenced. Combining related se-quences in GenBank, phylogenetic trees of Hexapoda were constructed by MP method using a crustacean Artemia salina as an outgroup. The results indicated that: (i) the integrated data of 18SrDNA and 28SrDNA could provide better phylogenetic information, which well supported the monophyly of Diplura; (ii) Diplura had a close phylogenetic relationship to Protura with high boot-strap support.

关 键 词：Diplura  Hexapoda  18SrRNA gene  28SrRNA gene  phylogeny. 

分 类 号：Q963[生物学—昆虫学]