Two-peak phenomena and formation origin in micellar electrokinetic capillary chromatography  

作　　者：陈冠华 杨更亮 田益玲 陈义 

机构地区：[1]College of Chemistry & Environmental Science, Hebei University, Baoding 071002,China [2]College of Life Science, Hebei Agricultural University, Baoding 071001, China [3]College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China [4]Molecular Science Center, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China

出　　处：《Science China Chemistry》2003年第5期473-480,共8页中国科学（化学英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Grant No.20075005); the Natural Science Foundation of Hebei Province,China(Grant Nos.200077 and 202096).

摘　　要：The formation origin of two peaks in micellar electrokinetic capillary chromatography by using cetyltrimethylaminium bromide (or sodium dodecyl sulfate) as pseudo stationary phase is studied. It is pointed out that two peaks may appear for one component in certain conditions. Experiments show that the relative areas of the two peaks of the corresponding component depend on the time and temperature of reaction between the analyte and the surfactant, and the concentration of surfactant in the sample solution. One of the two peaks increase with the increase of surfactant concentration in the sample solution while reverse for another peak. Temperature can accelerate the reaction process. This means that the interaction between analyte and surfactant is a slow process, and a stable substance can be produced from the interaction and leads to the formation of two peaks. The standpoint is confirmed by the infrared and nuclear magnetic resonance spectra of the product from the reaction between cetyltrimethylaminium bromide and m-hydroxyl benzoic acid.The formation origin of two peaks in micellar electrokinetic capillary chromatography by using cetyltrimethylaminium bromide (or sodium dodecyl sulfate) as pseudo stationary phase is studied. It is pointed out that two peaks may appear for one component in certain conditions. Experiments show that the relative areas of the two peaks of the corresponding component depend on the time and temperature of reaction between the analyte and the surfactant, and thconcentration of surfactant in the sample solution. One of the two peaks increase with the increase of surfactant concentration in the sample solution while reverse for another peak. Temperature can accelerate the reaction process. This means that the interaction between analyte and surfactant is a slow process, and a stable substance can be produced from the interaction and leads to the formation of two peaks. The standpoint is confirmed by the infrared and nuclear magneticresonance spectra of the product from the reaction between cetyltrimethylaminium bromide and m-hydroxyl benzoic acid.

关 键 词：TWO peaks  interaction  MICELLAR ELECTROKINETIC CAPILLARY chromatography  surfactant. 

分 类 号：O658.9[理学—分析化学]