SFE-CGC法测定七宝美髯胶囊中补骨脂素和异补骨脂素的含量  

SFE-CGC applied to determination of the psoralen and isopsoralen in capsulae Qibao Meiran

作　　者：邢旺兴[1] 王晓明[2] 陈斌[3,1] 陈斌[3,1] 吴玉田 

机构地区：[1]解放军第117医院,杭州310013 [2]南京军区联勤部卫生信息管理中心,南京210016 [3]第二军医大学药学院,上海200433

出　　处：《东南国防医药》2003年第2期85-87,共3页Military Medical Journal of Southeast China

摘　　要：目的 建立中成药七宝美髯胶囊中有效成分补骨脂素和异补骨脂素的含量测定方法。方法 采用超临界流体萃取(SFE)技术和毛细管气相色谱(CGC)非在线联用技术测定补骨脂素和异补骨脂素的含量,HP-5毛细管柱,FID检测器,蒽为内标。结果 SFE-CGC法测定七宝美髯胶囊中补骨脂素和异补骨脂素简便快速,结果准确可靠,加样回收率分别为97,76％(RSD2.31％),101.21％(RSD2.12％)。结论 SFE-CGC法可以作为七宝美髯颗粒质量控制的方法之一。Objective The method has been developed for quantitative analysis of effective components psoralen and isopsoralen in Chinese patent medicines, capsulae Qibao Meiran by SFE coupled with CGC. Methods Psoralen and isopsoralen were extracted by SFE and internal standard method was used for the quantitative determination using HP-5 capillary column, FID detector and anthracene as the internal standard. Results This method is quick, convenient, precise and accurate, which added sample recoveries of psoralen and iaopsoralen are 97. 76% (RSD 2. 31%) and 101. 21%(RSD 2. 12%) respectively. Conclusions The SFE-CGC method was suitable for the quality control of capsulae Qibao Meiran.

关 键 词：超临界流体萃取 七宝美髯胶囊 补骨脂素 异补骨脂素 

分 类 号：R286.0[医药卫生—中药学]