5′-硝基-水杨基荧光酮与头孢哌酮的显色反应  

Color reaction study and application between 5-nitro salicylfluorone and cefoperazone

作　　者：邢高娃[1] 敖登高娃[1] 刘俊轶[1] 

机构地区：[1]内蒙古大学化学化工学院,呼和浩特010021

出　　处：《分析试验室》2010年第9期32-34,共3页Chinese Journal of Analysis Laboratory

基　　金：内蒙古教育厅基金项目(2009MS0206)资助

摘　　要：采用分光光度法,以5′-硝基-水杨基荧光酮(5′-NSAF)为显色剂,研究了5′-NSAF与头孢哌酮(CPZ)的络合物的可见光谱性质及反应条件。实验结果表明:头孢哌酮在6.68~133.6μg/mL范围内遵循比尔定律,回归方程为:A=0.0031ρ+0.023(ρ:μg/mL),检出限6.27μg/mL,表观摩尔吸光系数1.89×104L.mol-1.cm-1,相关系数为r=0.9970,并探讨了其反应机理。方法已用于尿样及生物样品中头孢哌酮含量的测定。The visible spectral characteristic and the reaction condition of complex of cefoperazone(CPZ) and 5-nitro salicylfluorone(5′-NSAF) were investigated by spectrophotometry which 5-nitro salicylfluorone as a developer.The results showed that the concentration of CPZ obeyed the Beer’s law in the range of 6.68～133.6 μg/mL and the regression equation was A=0.0031 ρ+0.023(ρ:μg/mL) with the correlation coefficient R of 0.9970,the apparent molar absorptivity was 1.89×104 L·mol-1·cm-1 and the detection limit was 6.27 μg/mL.Furthermore,the reaction mechanism was also explored elementarily.The proposed method has been successfully applied to the determination of CPZ in urine and biological samples,and the results were satisfying.

关 键 词：5′-硝基-水杨基荧光酮 头孢哌酮 分光光度法 

分 类 号：R115[医药卫生—公共卫生与预防医学]