高效液相色谱-二极管阵列检测法测定L-丙氨酸与L-氨基丙醇  

Determination of L-alanine and L-alaninol by high performance liquid chromatography with photodiode array detectors

作　　者：王永秋[1] 赵慧[1] 

机构地区：[1]淮北煤炭师范学院化学与材料科学学院,淮北235300

出　　处：《分析试验室》2010年第9期44-46,共3页Chinese Journal of Analysis Laboratory

基　　金：安徽高校省级自然科学研究项目(KJ2008B179)资助

摘　　要：建立了柱前衍生同时测定L丙-氨酸、L氨-基丙醇的高效液相色谱方法。色谱条件为:Shim-pack VP-ODS柱(150 mm×4.6 mm.ID.,5μm);二极管阵列检测器;流动相为甲醇-磷酸盐缓冲溶液(NaH2PO4浓度为0.02 mol/L,pH 4.00 H3PO4调节)(体积比为30∶70),流速为0.5 mL/m in,检测波长为222 nm,柱温为30℃。L丙-氨酸与L氨-基丙醇的线性范围分别为2.30~178 mg/L和9.3~8690 mg/L;检出限分别为1.1 mg/L和1.6 mg/L;日内、日间测定的相对标准偏差(RSD)分别为0.22%、0.46%和0.02%、0.03%。A precolumn derivatization method to determine L-alanine and L-alaninol by high performance liquid chromatography(HPLC) was established.The HPLC with photodiode array detection(PAD) was performed on a Shim-pack VP-ODS column(150 mm×4.6 mm i.d.,5 μm).The mobile phase was CH3OH-0.02 mol/L NaH2PO4(30∶70 v/v),of which NaH2PO4 solution was adjusted to pH 4.00 with H3PO4.The flow rate was 0.50 mL/min at the column temperature of 30 ℃ and the detection wave length was 222 nm.The linear range of L-alanine was 2.30～178 μg/mL while that of L-alaninol was 9.3～8690 μg/mL.The detection limits of L-alanine and L-alaninol were 1.1 μg/mL and 1.6 μg/mL respectively.The relative standard deviations(RSDs) of inter-and intra-day precision experiments were 0.22%、0.46% and 0.003%、0.02% accordingly.

关 键 词：高效液相色谱 L丙-氨酸 L氨-基丙醇 柱前衍生 

分 类 号：TQ207.4[化学工程—有机化工]