高效液相色谱-化学发光法测定复方吲哚美辛酊及人体尿液中的吲哚美辛  

Determination of indomethacin in compound indomethacin tincture and human urine samples by High-performance Liquid Chromatography with chemiluminescence detection

作　　者：龙星宇[1] 陈福南[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《分析试验室》2010年第9期57-60,共4页Chinese Journal of Analysis Laboratory

摘　　要：在酸性条件下,硫酸铈将分别氧化钌(II)联吡啶和吲哚美辛,产生的氧化产物会相互反应而发生化学发光。据此建立通过HPLC分离、用化学发光检测器测定吲哚美辛的方法。在优化的实验条件下,测定吲哚美辛的线性范围为22.95~61.20μg/mL,检出限(3σ)为6.12μg/mL,相对标准偏差为2.7%(n=11)。方法可用于生物药品复方吲哚美辛酊及人体尿液中吲哚美辛的含量。A chemiluminescence method for the determination of indomethacin was described.It was based on the chemiluminescence produced during the oxidation of Indomethacin and tris(2,2-bipyridyl)ruthenium(II) by Ce(IV) in acidic solution.The CL intensity was linear in the range of 22.95～61.20 μg/mL with the detection limit of 6.12 μg/mL.The relative standard deviation was 2.7%(n=11).This method was applied to the analysis of indomethacin in compound indomethacin tincture and human urine samples.

关 键 词：高效液相色谱 化学发光 复方吲哚美辛酊 人体尿液 吲哚美辛 

分 类 号：R96[医药卫生—药理学]