高效液相色谱法用于普萘洛尔与β_2肾上腺素受体结合实验研究  

Study on propranolol binding with β2-adrenergic receptor from erythrocyte membrane by high performance liquid chromatography

作　　者：蒋心惠[1] 杨俊卿 周岐新[1] 

机构地区：[1]重庆医科大学药学院,重庆400016

出　　处：《分析试验室》2010年第9期70-73,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(No.30572353)项目资助

摘　　要：建立了用高效液相色谱检测红细胞混悬液中盐酸普萘洛尔的方法并用于红细胞膜β2受体-配体结合实验研究。通过1500 r/m in离心5 m in的方法将试样中游离普萘洛尔和含受体-普萘洛尔复合物的红细胞分离,采用HypersilBDS?C18柱(150 mm×4.6 mm,5μm)为分析色谱柱;流动相为20 mmol/LKH2PO4溶液(其中含0.25%三乙胺,pH 5.1)?甲醇(65∶35,V/V),流速为1.0 mL/m in;276 nm波长处检测;柱温30℃;进样量20μL。普萘洛尔在5~150 ng/mL范围内线性关系良好,相关系数为0.9998。样品测定的重复性相对标准偏差为1.05%;检出限为1.5 ng/mL。样品测定结果表明普萘洛尔的特异性结合量随其加入量的增加而增大,然后达到饱和。该方法可用于受体配体结合实验的研究。An effective high performance liquid chromatographic method with hypersil C18 column,0.020 mol / L potassium dihydrogen phosphate solution(which contains 0.25% triethylamine,pH 5.1)-methanol(65∶35,V/V) as mobile phase and detection at UV 276 nm wavelength has been developed for the determination of propranolol in Erythrocyte suspensions which for investigate propranolol binding to β2 receptor.The flow rate was 1.0 mL/min and the column temperature was 30 ℃.The linear range was 5.0～150 ng/mL and the correlat...

关 键 词：普萘洛尔 红细胞膜受体 Β2肾上腺素受体 高效液相色谱 

分 类 号：R965.2[医药卫生—药理学]