多壁碳纳米管固载亚甲基蓝复合膜修饰玻碳电极测定己烯雌酚  

Determination of diethylstilbestrol based on methylene blue immobilized on Multi-walled carbon nanotubes modified glassy carbon electrodes

作　　者：刘成伦[1] 张柳[1] 杨琦[1] 张晓敏[1] 王茂君[1] 

机构地区：[1]重庆大学化学化工学院,重庆400044

出　　处：《分析试验室》2010年第9期74-77,共4页Chinese Journal of Analysis Laboratory

基　　金：重庆市科委自然科学基金(CSTC;2007BB0200);重庆大学"211"三期创新人才培养计划(S-09103)资助项目

摘　　要：提出了多壁碳纳米管-聚亚甲基蓝(MWNT/PMB)复合膜修饰玻碳电极检测己烯雌酚(DES)的方法,考察了缓冲液、pH、扫描速度等实验参数对测定的影响。在0.2 mol/L的磷酸缓冲液(pH7.0)中,DES在复合膜电极上出现了一个明显的氧化峰,峰电位在0.197 V处。与裸电极相比较,修饰后的电极能显著提高DES的氧化峰电流并降低其氧化电位,表明MWNT/PMB复合膜对DES有电催化作用。峰电流与己烯雌酚浓度在3.73×10-11~3.73×10-8mol/L范围内呈线性关系,检出限为2×10-11mol/L;用此法对己烯雌酚药片的含量进行了测定,测定结果与标示相符。A method for the determination of DES at glassy carbon electrodes modified by MWNT/PMB composite films was proposed,and various factors influencing experimental results such as buffer solution,pH and scan rate were investigated.In a 0.2 mol/L PBS buffer solution(pH7.0),DES exhibited a well-defined and sensitive oxidation peak at the MWNT/PMB/GCE.The oxidation peak potential was at 0.197 V.Compared with the bare electrode,the oxidation peak current of DES increased remarkably and the peak potential shifted negatively at the MWNT/PMB/GCE,which suggested that the MWNT/PMB/GCE exhibited catalytic activity to the oxidation of DES.The oxidation peak current was linear with the concentration of DES over the range from 3.73×10-11 mol/L to 3.73×10-8 mol/L.The detection limit was 2×10-11 mol/L,The proposed method was used for the determination of DES in tablets and the results were consistent with the marked those.

关 键 词：多壁碳纳米管 亚甲基蓝 己烯雌酚 电催化 

分 类 号：R927.2[医药卫生—药学]