高效液相色谱手性拆分药物对映体机理研究  

Study on enantioseparation mechanism of drug enantiomers by HPLC

作　　者：唐琴[1] 陈先勇[1] 宋航[2] 

机构地区：[1]湖北民族学院化学与环境工程学院,恩施445000 [2]四川大学化工学院,成都610065

出　　处：《分析试验室》2010年第9期29-31,共3页Chinese Journal of Analysis Laboratory

基　　金：湖北省教育厅项目(B20082903);四川省应用基础研究基金项目(04JY029-083-3)资助

摘　　要：于Whelk-O1(R,R)手性固定相上直接拆分药物萘普生和丙卡特罗。两药物的对映体在该手性固定相上均可以实现基线分离,分离因子达1.4以上。根据相关手性色谱分离热力学数据,进行了手性识别机理研究,并据此成功预测了药物克仑特罗对映体在Whelk-O1(R,R)手性柱上的拆分。The enantiomers of drugs naproxen and procaterol have been separated on Whelk-O1 chiral stationary phase.The results show that the baseline enantiomer resolution of this two chiral drugs on Whelk-O1 have been achieved with separation factor α above 1.4,when using different mobile phase compositions of 2-propanol-hexane at different column temperatures and at a flow rate of 1.5 mL/min.The mechanism of chiral recognition processes has been studied based on the related thermodynamic data of enantiomertric separation by chromatography.Clenbuterol enantiomers have been resolved on this chiral column according to it.

关 键 词：高效液相色谱 手性拆分 萘普生 丙卡特罗 机理研究 

分 类 号：R917[医药卫生—药物分析学]