Fertilization in Torenia fournieri:actin organization and nuclear behavior in the central cell and primary endosperm  

作　　者：袁明 傅缨 王凤 黄炳权 徐是雄 Peter K.Hepler 

机构地区：Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China[1] Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China Research Center for Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China[2] Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China[3] Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China[4] Department of Biology, University of Massachusetts, Amberst, Massachusetts 01003, USA[5]

出　　处：《Science China(Life Sciences)》2002年第2期211-224,共16页中国科学（生命科学英文版）

基　　金：This research was supported by a RGC grant from the Research Grants Council of Hong Kong anda CRCG grant from University of Hong Kong to B. Q. H, a CRCG grant to S. Y. Z, NSF grant No. MCB-9601087 to P. K. H., and the National Key Basic Research Projec

摘　　要：Studies of the living embryo sacs of Torenia fournieri reveal that the actin cytoskeleton undergoes dramatic changes that correlate with nuclear migration within the central cell and the primary endosperm. Before pollination, actin filaments appear as short bundles randomly distributed in the cortex of the central cell. Two days after anthesis, they become organized into a distinct actin network. At this stage the secondary nucleus, which is located in the central region of the central cell, possesses an associated array of short actin filaments. Soon after pollination, the actin filaments become fragmented in the micropylar end and the secondary nucleus is located next to the egg apparatus. After fertilization, the primary endosperm nucleus moves away from the egg cell and actin filaments reorganize into a prominent network in the cytoplasm of the primary endosperm. Disruption of the actin cytoskeleton with latrunculin A and cytochalasin B indicates that actin is involved in the migration of the nucleus in the central cell. Our data also suggest that the dynamics of actin cytoskeleton may be responsible for the reorganization of the central cell and primary endosperm cytoplasm during fertilization.Studies of the living embryo sacs of Torenia fournieri reveal that the actin cytoskeleton undergoes dramatic changes that correlate with nuclear migration within the central cell and the primary endosperm. Before pollination, actin filaments appear as short bundles randomly distributed in the cortex of the central cell. Two days after anthesis, they become organized into a distinct actin network. At this stage the secondary nucleus, which is located in the central region of the central cell, possesses an associated array of short actin filaments. Soon after pollination, the actin filaments become fragmented in the micropylar end and the secondary nucleus is located next to the egg apparatus. After fertilization, the primary endosperm nucleus moves away from the egg cell and actin filaments reorganize into a prominent network in the cytoplasm of the primary endosperm. Disruption of the actin cytoskeleton with latrunculin A and cytochalasin B indicates that actin is involved in the migration of the nucleus in the central cell. Our data also suggest that the dynamics of actin cytoskeleton may be responsible for the reorganization of the central cell and primary endosperm cytoplasm during fertilization.

关 键 词：actin  EMBRYO sac  central cell  Torenia. 

分 类 号：Q945[生物学—植物学]