The complete sequence of a sugarcane mosaic virus isolate causing maize dwarf mosaic disease in China  

作　　者：程晔 陈炯 陈剑平 

出　　处：《Science China(Life Sciences)》2002年第3期322-330,共9页中国科学（生命科学英文版）

基　　金：This work was supported by Zhejiang Provincial Foundation for Natural Sciences (RC9604), a special grant from Zhejiang Provincial Department of Science and Technology (001107557) ; the 10th Five-Year Research Programme of Zhejiang Province (011102181).

摘　　要：The complete sequence of a potyvirus from maize in Zhejiang Province was determined. The RNA was 9596 nucleotides long, excluding the 3'-poly (A) tail, and there was a single long open reading frame (ORF) of 9192 nts encoding a 346.1 ku polyprotein. The polyprotein had substantial amino acid sequence homology with those encoded by the RNAs of a Chinese isolate of sorghum mosaic virus (SrMV-C) and a Bulgarian isolate of maize dwarf mosaic virus, but it was most closely related to sugarcane mosaic virus (SCMV) isolates, for which only partial sequences have been published. According to the published criteria for distinguishing potyviruses, the sequence reported here is clearly a strain of SCMV, but it also showed a surprisingly high amino acid homology with SrMV-C in the HC-Pro, P3 and Cl proteins.The complete sequence of a potyvirus from maize in Zhejiang Province was determined. The RNA was 9596 nucleotides long, excluding the 3′-poly (A) tail, and there was a single long open reading frame (ORF) of 9192 nts encoding a 346.1 ku polyprotein. The polyprotein had substantial amino acid sequence homology with those encoded by the RNAs of a Chinese isolate of sorghum mosaic virus (SrMV-C) and a Bulgarian isolate of maize dwarf mosaic virus, but it was most closely related to sugarcane mosaic virus (SCMV) isolates, for which only partial sequences have been published. According to the published criteria for distinguishing potyviruses, the sequence reported here is clearly a strain of SCMV, but it also showed a surprisingly high amino acid homology with SrMV-C in the HC-Pro, P3 and CI proteins.

关 键 词：maize DWARF MOSAIC disease  SUGARCANE MOSAIC virus  genome organization  taxonomy. 

分 类 号：S432.41[农业科学—植物病理学]