Effect of thermal injury on LPS-mediated Toll signaling pathways by murine peritoneal macrophages:inhibition of DNA-binding of transcription factor AP-1 and NF-κB and gene expression of c-fos and IL-12p40  

作　　者：王兴宇 李昌麟 张劲松 冯卫国 苏踊跃 张宗梁 

出　　处：《Science China(Life Sciences)》2002年第6期613-622,共10页中国科学（生命科学英文版）

基　　金：This work was supported by the Ministry of Science and Technology of China

摘　　要：Thermal injury is able to induce the immune functional inhibition in the host, but the mechanisms are largely unknown. The available evidence indicates that macrophages play an important role in immune regulations and host defenses. Thus, studies of the effect of thermal injury on macrophages are essential. Transcriptional factors of AP-1 (activator protein-1) and NF-кB (nuclear factor-кB) are critical members of signal transduction pathways. These transcriptional factors have been shown to be necessary for a variety of expressions of cytokine genes. In this investigation, the effect of thermal injury on AP-1 and NF-кB was studied using a model for the murine thermal injury. Our findings indicated that thermal injury mediated the inhibition of AP-1 and NF-кB DNA binding activities and c-fos and IL-12 p40 gene expressions in response to LPS.Thermal injury is able to induce the immune functional inhibition in the host, but the mechanisms are largely unknown. The available evidence indicates that macrophages play an important role in immune regulations and host defenses. Thus, studies of the effect of thermal injury on macrophages are essential. Transcriptional factors of AP-1 (activator protein-1) and NF-κB (nuclear factor-κB) are critical members of signal transduction pathways. These transcriptional factors have been shown to be necessary for a variety of expressions of cytokine genes. In this investigation, the effect of thermal injury on AP-1 and NF-κB was studied using a model for the murine thermal injury. Our findings indicated that thermal injury mediated the inhibition of AP-1 and NF-κB DNA binding activities and c-fos and IL-12 p40 gene expressions in response to LPS.

关 键 词：thermal injury  AP-1  NF-кB  macrophages. 

分 类 号：Q493[生物学—生理学]