Construction of a new plant expression vector containing two insect resistant genes and its expression in transgenic tobacco plants  

作　　者：GUO Hongnian, QIN Hongmin, CHEN Xiaoying, LI Changqing, LU Rui and TIAN Yingchuan(Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China) 

出　　处：《Progress in Natural Science:Materials International》2002年第5期347-352,共6页自然科学进展·国际材料（英文版）

基　　金：Supported by the National "863" High-Tech Projects (Grant No. Z-17-01-01 and 2001AA212071); partly by the World Laboratory of the International Center of Science and Culture (ICSC)

摘　　要：A new plant expression vector (pBS29K-BA) containing two insect resistant genes, a synthetic chimeric gene BtS29K encoding the activated insecticidal protein Cry1Ac and a gene API-BA encoding the arrowhead (Sagittaria sagittifolia L.) proteinase inhibitor (API) A and B, is constructed. Transgenic tobacco plants expressing these two genes are obtained through Agrobacterium-mediated transformation of tobacco leaf discs. The average expression levels of Cry1Ac and API-BA proteins in transgenic plants are of 3.2 μg and 4.9 μg per gram fresh leaf respectively. The results of insecticidal assay of transgenic plants indicate that the pBS29K-BA transformed plants are more resistant to insect damage than the plants expressing the Cry1Ac gene or API-BA gene alone.A new plant expression vector (pBS29K-BA) containing two insect resistant genes, a synthetic chimeric gene BtS29K encoding the activated insecticidal protein Cry1Ac and a gene API-BA encoding the arrowhead (Sagittaria sagittifolia L.) proteinase inhibitor (API) A and B, is constructed. Transgenic tobacco plants expressing these two genes are obtained through Agrobacterium-mediated transformation of tobacco leaf discs. The average expression levels of Cry1Ac and API-BA proteins in transgenic plants are of 3.2 mug and 4.9 mug per gram fresh leaf respectively. The results of insecticidal assay of transgenic plants indicate that the pBS29K-BA transformed plants are more resistant to insect damage than the plants expressing the Cry1Ac gene or API-BA gene alone.

关 键 词：cry1Ac  ARROWHEAD PROTEINASE inhibitor  insect-resistant TRANSGENIC plants. 

分 类 号：Q943.2[生物学—植物学]