Construction of human and mouse brain cDNA libraries and isolation of full-length cDNAs  

作　　者：ZHOU Yan, HU Songnian, HUANG Xiaowei, ZHOU Haijun, TAN Xinyu, PENG Xiaozhong, QIANG Boqin and YUAN Jiangang( State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Chinese National Human Genome Center, Beijing 100005, China) 

出　　处：《Progress in Natural Science:Materials International》2002年第6期477-480,共4页自然科学进展·国际材料（英文版）

基　　金：Supported by the Major State Basic Research Development Program of China (G19988051000) ;the National Natural Science Foundation of China (Grant No. 39830070)

摘　　要：cDNA libraries from aborted human 3-month fetal brain,adult rat and mouse brain were constructed by using a yZAP express cDNA library construction kin.Low molecular weight fragments of the second strand cDNASA were removed by flowing through the Sepharose CL-4B column and the frractionated long,Middle,Short fragments and the combined fragments weire respectively inserted into clone vectors to construct the cDNA libraries of the brain of human 3-month fetus.The 5'ends of 1200 clones from each of human fetal brain cDNA libraries were sequenced.A total of 894 ESTs were obtained and some full-length clones were squenced.By andalyaing the se-quences,12 novel full-length cDNAs were obtained.cDNA libraries from aborted human 3-month fetal brain, adult rat and mouse brain were constructed by using a ZAP express cDNA library construction kit. Low molecular weight fragments of the second strand cDNAs were removed by flowing through the sepharose CL-4B column and the fractionated long, middle, short fragments and the combined fragments were respectively inserted into clone vectors to construct the cDNA libraries of the brain of human 3-month fetus. The 5' ends of 1200 clones from each of human fetal brain cDNA libraries were sequenced, A total of 894 ESTs were obtained and some full-length clones were sequenced. By analyzing the sequences, 12 novel full-length cDNAs were obtained.

关 键 词：CDNA library brain EST. 

分 类 号：Q523[生物学—生物化学]