Cloning of plastid division gene GlFtsZ from Gentiana lutea and its expression during petal development  

作　　者：HE Yikun , ZHU Changfu , WANG Dong, KONG Dongdong and SUN Jingsan(1. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2. Department of Biology, Capital Normal University, Beijing 100037, China 3. College of Life Science, Northeast Normal University, Changchun 130024, China) 

出　　处：《Progress in Natural Science:Materials International》2002年第8期592-597,共6页自然科学进展·国际材料（英文版）

基　　金：Supported by the Senior Visitor Project of Chinese Academy of Sciences, the National Natural Science Foundation of China (Grant No. 39970356) ; the Natural Science Foundation of Beijing (Grant No. 5992003); A part of work was carried out in Iwate Biote

摘　　要：A full-length cDNA of GlFtsZ was isolated by screening the cDNA library of Gentiana lutea. Analysis of the deduced amino acid sequence encoded by GlFtsZ indicated that GlFtsZ protein possesses the typical conservative motifs existed in all FtsZ proteins. The existence of putative plastid transit peptide in its N-terminus suggested that GlFtsZ might function inside of plastids. With the deve- lopmental process of petals of Gentiana lutea, the expression of plastid division gene GlFtsZ declined gradually, whereas the expression of carotenoids biosynthesis gene Zds increased obviously; meanwhile, in contrast to the increment of carotenoids, the content of chlorophyll in petals decreased sharply. The chloroplasts turned into chromoplasts, and the color of petals also turned from green to golden. All of these results suggested that the expression of GlFtsZ is accompanied with the development and differentiation of plastids.A full-length cDNA of GlFtsZ was isolated by screening the cDNA library of Gentiana lutea. Analysis of the deduced amino acid sequence encoded by GlFtsZ indicated that GlFtsZ protein possesses the typical conservative motifs existed in all FtsZ proteins. The existence of putative plastid transit peptide in its N-terminus suggested that GlFtsZ might function inside of plastids. With the developmental process of petals of Gentiana lutea, the expression of plastid division gene GlFtsZ declined gradually, whereas the expression of carotenoids biosynthesis gene Zds increased obviously; meanwhile, in contrast to the increment of carotenoids, the content of chlorophyll in petals decreased sharply. The chloroplasts turned into chromoplasts, and the color of petals also turned from green to golden. All of these results suggested that the expression of GlFtsZ is accompanied with the development and differentiation of plastids.

关 键 词：FTSZ gene  plastids  chromoplasts  GENTIANA lutea. 

分 类 号：Q785[生物学—分子生物学]