Cloning of Promoter of Chinese Bean GRP 1.8 Gene and Characterization of Its Function in Transgenic Tobacco Plants  

Cloning of Promoter of Chinese Bean GRP 1.8 Gene and Characterization of Its Function in Transgenic Tobacco Plants

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作  者:LU Hai JIANG Xiang ning LI Feng lan ZENG Qing yin LIU Wei GOU Xiao jun 

机构地区:[1]Experimental Center of Forest Biology,College of Plant Sciences,Beijing Forestry University,Beijing 100083,P.R.China [2]Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education,Jilin University,Changchun 130023,P.R.China

出  处:《Chemical Research in Chinese Universities》2002年第3期290-293,共4页高等学校化学研究(英文版)

基  金:Supported by the National Natural Science Foundation of China(No.39730 35 0 ) .

摘  要:In order to learn the expression pattern of GRP1 8(glycine rich protein) gene promoter in transgenic plants and to explore its potential application in plant genetic engineering for vascular specific expression of interested genes, GRP 1 8 promoter was amplified by PCR from Chinese bean genomic DNA. The intermediate vector was constructed by inserting vascular specific expression promoter of GRP 1 8 gene in vector pBI 101. The regenerated tobacco plants obtained were analyzed by PCR to select the putative transgenic plants. The histochemical localization of GUS( β D glucosidase) activity indicates that as for that of GRP 1 8 promoter we can confer the vascular specific expression of GUS gene.In order to learn the expression pattern of GRP1 8(glycine rich protein) gene promoter in transgenic plants and to explore its potential application in plant genetic engineering for vascular specific expression of interested genes, GRP 1 8 promoter was amplified by PCR from Chinese bean genomic DNA. The intermediate vector was constructed by inserting vascular specific expression promoter of GRP 1 8 gene in vector pBI 101. The regenerated tobacco plants obtained were analyzed by PCR to select the putative transgenic plants. The histochemical localization of GUS( β D glucosidase) activity indicates that as for that of GRP 1 8 promoter we can confer the vascular specific expression of GUS gene.

关 键 词:Chinese Bean Promoter of GRP 1 8 gene Transgenic tobacco plants 

分 类 号:Q943.2[生物学—植物学]

 

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