Biosynthesis of Unsaturated Fatty Acids via Mortierella Isabellina Cultivated in a Medium Containing Butanol  

作　　者：XIAN Mo YAN Ji chang CHENG Tie xin KANG Yi jian LI Wen xing BI Ying li ZHEN Kai ji 

机构地区：[1]College of Chemistry,Jilin University,Changchun 130023,P.R.China [2]College of Chemistry,Northeast Normal University,Changchun 130024,P.R.China [3]Department of Chemical Engineering,Qingdao Chimical Industry Institute,Qingdao 266042,P.R.China

出　　处：《Chemical Research in Chinese Universities》2002年第3期303-306,共4页高等学校化学研究(英文版)

基　　金：Supported by the National Natural Science Foundation of China(No.2 98730 18)

摘　　要：Mortierella isabellina was found to accumulate large amounts of unsaturated fatty acids when it was grown in a medium containing butanol(5 g/L) and yeast extract(10 g/L) and cultivated at 25 ℃ for 5 d during which additional feeding butanol of 2 g/L was fed after being cultivated for 48 h . The resultant mycelial lipids accounted for 40 1% of the dry mycelia, while about 34% of butanol in the medium was converted. The mycelial lipids contained four kinds of unsaturated fatty acids, i.e ., palmitoleic(4 9%), oleic(54 1%), linoleic(10 4%) and linolenic(5 4%) acids. Those accounted for 74 8% of the total fatty acids. The effects of the culture conditions, such as cultivation temperature, initial pH of the medium and additional feeding butanol in the course of cultivation, on the production of mycelial lipids by M. isabellina were studied.Mortierella isabellina was found to accumulate large amounts of unsaturated fatty acids when it was grown in a medium containing butanol(5 g/L) and yeast extract(10 g/L) and cultivated at 25 ℃ for 5 d during which additional feeding butanol of 2 g/L was fed after being cultivated for 48 h . The resultant mycelial lipids accounted for 40 1% of the dry mycelia, while about 34% of butanol in the medium was converted. The mycelial lipids contained four kinds of unsaturated fatty acids, i.e ., palmitoleic(4 9%), oleic(54 1%), linoleic(10 4%) and linolenic(5 4%) acids. Those accounted for 74 8% of the total fatty acids. The effects of the culture conditions, such as cultivation temperature, initial pH of the medium and additional feeding butanol in the course of cultivation, on the production of mycelial lipids by M. isabellina were studied.

关 键 词：Mortierella isabellina BUTANOL Unsaturated fatty acid BIOSYNTHESIS 

分 类 号：O623[理学—有机化学]