Analysis of Mitochondrial DNA from the Ancient Tombs of Turfan  

作　　者：CUI Yin-qiu DUAN Ran-hui LIU Shu-bai JI Chao-neng ZHU Hong LI Wei MAO Yu-min ZHOU Hui 

机构地区：[1]Ancient DNA Laboratory,College of Life Science,Jilin University,Changchun 130023,P.R.China [2]State Key Laboratory of Genetic Engineering,School of Life Science,Fudan University,Shanghai 200433,P.R.China

出　　处：《Chemical Research in Chinese Universities》2002年第4期419-423,共5页高等学校化学研究(英文版)

基　　金：Supported by Fund for the Important Project of the Science and Technology Department;Ministry of Education

摘　　要：MtDNA was successfully extracted from ten individual bones (femurs) in the tombs of ancient Jushi in Turfan basin, dated back to the year about 3 000-2 500 years ago. By means of four overlapping primers, we got nucleotide sequence of the 218bp length. Ancient mtDNA was analyzed by the sequencing of hypervariable region Ⅰ of the mtDNA control region. The result shows that 9 haplotypes with 24 polymorphic sites were obtained. The phylogenetic analysis indicated that Mongolians and Altai are the population genetically closest to the Jushi groups and Jushi mtDNA pool being an admixture of eastern Asian and European lineages. So our preliminary data imply that an ancient mingling of Euro-Asian population had existed in Turfan basin prior to the early Iron Age.MtDNA was successfully extracted from ten individual bones (femurs) in the tombs of ancient Jushi in Turfan basin, dated back to the year about 3 000-2 500 years ago. By means of four overlapping primers, we got nucleotide sequence of the 218bp length. Ancient mtDNA was analyzed by the sequencing of hypervariable region Ⅰ of the mtDNA control region. The result shows that 9 haplotypes with 24 polymorphic sites were obtained. The phylogenetic analysis indicated that Mongolians and Altai are the population genetically closest to the Jushi groups and Jushi mtDNA pool being an admixture of eastern Asian and European lineages. So our preliminary data imply that an ancient mingling of Euro-Asian population had existed in Turfan basin prior to the early Iron Age.

关 键 词：Ancient DNA SEQUENCE Phylogenetic analysis 

分 类 号：Q52[生物学—生物化学]