盐析分相微萃取—高效液相色谱法测定水中取代苯  

Determination of Substituted Benzenes in Water by Salting Out Phase Separation Microextraction-High Performance Liquid Chromatography

作　　者：周建科[1] 龙堃[1] 彭静[1] 

机构地区：[1]河北大学理化分析中心河北省分析科学技术重点实验室,河北保定071002

出　　处：《化工环保》2010年第2期180-183,共4页Environmental Protection of Chemical Industry

摘　　要：建立了测定水中三种取代苯的盐析分相微萃取—高效液相色谱分析方法。实验结果表明,当异丁醇萃取剂加入量为300μL、(NH_4)_2SO_4加入量为14 g、流动相V(甲醇):V(水)为80:20、检测波长为220 nm时,邻氯苯酚、氯苯、乙苯的工作曲线均呈现良好线性(相关系数大于0.999 9),方法检出限分别为0.07,0.2,9.0μg/L,加标回收率为71.66%～92.96%,相对标准偏差为1.17%～5.17%。The method of salting out phase separation microextraction-high performance liquid chromatography was established to determine three substituted benzenes in water.The experimental results show that:When the dosage of extractant isobutyl alcohol is 300μL,the dosage of （NH4）2SO4 is 14 g,the ratio of V（methanol）:V（water） in mobile phase is 80:20 and the detection wavelength is 220 nm,the work curves of o-chlorophenol,chlorobenzene and ethylbenzene exhibite good linearity with the correlation coefficient above 0.999 9;The detection limits are 0.07μg/L for o-chlorophenol,0.2μg/L for chlorobenzene and 9.0 g/L for ethylbenzene;The recoveries of standards are 71.66%-92.96%and the relative standard deviations are 1.17%-5.17%.

关 键 词：盐析 分相 微萃取 高效液相色谱 邻氯苯酚 氯苯 乙苯 分析方法 

分 类 号：X832[环境科学与工程—环境工程]