枯草芽孢杆菌Bs-15产嗜铁素条件及其对甜椒的防病促生效应  

Study on factors influencing the siderophore production of Bacillus subtilis Bs-15 and effects of Bs-15 on disease control and growth promotion of sweet pepper

作　　者：余贤美[1] 周广芳[1] 辛力[1] 

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《农药学学报》2010年第2期135-141,共7页Chinese Journal of Pesticide Science

基　　金："十一五"国家科技支撑计划资助项目(2008BAD92B03)

摘　　要：研究了不同培养条件对枯草芽孢杆菌Bs-15嗜铁素产量的影响。结果表明,以改良MM培养基为基础培养基,以葡萄糖为碳源,以色氨酸为外源氨基酸,在pH7.2、装液量100mL/250mL、37℃、200r/min下培养,可获得较高产量的嗜铁素。盆钵试验结果表明:若同时接种Bs-15和枯萎病菌,Bs对枯萎病菌的防效为44.44%;若施用Bs-152d后再接种枯萎病菌,防效可达56.94%。说明Bs-15可能通过产生系统诱导抗性增强了对枯萎病的防效。研究结果还表明,Fe离子可大大降低Bs-15对枯萎病的防治效果,防效仅为12.50%和23.43%。另外,经Bs-15浸种处理播种后第14,21,28和40d的甜椒苗比对照增高27.24%～54.53%;与对照相比,处理组中有50%的甜椒开花时间提前了17.26d,平均单果重和单株产量分别提高36.92%和49.68%。表明枯草芽孢杆菌Bs-15在甜椒枯萎病防治及生长促进作用方面具有较好的效果。The influence of culture conditions on the siderophore production of Bacillus subtilis Bs-15 was studied.The results showed that Bs-15 could yield high content of siderophore when inoculated 100 mL modified MM medium(pH 7.2)with glucose as carbon source and tryptophan as nitrogen source,and shaking under 200 r/min in 250 mL flask at 37℃.The effects of B.subtilis Bs-15 on the biocontrol of Fusarium wilt and growth promotion of sweet pepper was studied by pot experiment.The results showed that Bs-15 decreased...

关 键 词：枯草芽孢杆菌 甜椒 镰刀菌枯萎病 生物防治 植物促生作用 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]