一株虫生真菌杀线虫活性代谢产物的分离与结构鉴定  

Isolation and identification of the nematicidal secondary metabolites from one strain of entomogenous fungi

作　　者：张永刚[1] 袁文鹏[1] 夏雪奎[1] 刘新[1] 孟秀梅[1] 王小军[1] 张绵松[1] 刘昌衡[1] 

机构地区：[1]山东省科学院中日友好生物技术研究中心山东省应用微生物重点实验室,济南250014

出　　处：《农药学学报》2010年第2期225-228,共4页Chinese Journal of Pesticide Science

基　　金：国家重点基础研究发展规划("973"计划)资助项目(2004CB719601);山东省科学院博士基金项目(ZBS2010Y)

摘　　要：以生物活性为导向,利用硅胶、凝胶柱层析法以及高效液相色谱等技术对一株虫生真菌拟青霉菌Paecilomycessp.的活性次生代谢产物进行了研究。从其活性组份中共分离得到2个杀线虫活性次生代谢产物,经质谱和核磁共振等波谱分析方法鉴定其结构分别为脑苷酯类化合物Cerebroside A(1)和Cerebroside B(2)。活性测定结果显示,这2个化合物均具有杀松材线虫Bursaphelenchus xylophilus活性,其中化合物1在质量浓度分别为1000,100和10μg/mL下对松材线虫的致死率分别为100%,100%和11.1%。By application of different separation techniques,such as silica gel,Sephadex LH-20 column chromatography,and reversed-phase high performance liquid chromatography,two nematicidal secondary metabolites were isolated from the fermentation broth of one strain of entomogenous fungi Paecilomyces sp.The structures of these compounds were elucidated as Cerebroside A(1)and Cerebroside B(2)primarily by analysis of their NMR spectroscopic data.Bioassay tests showed that the two compounds had moderate nematicidal act...

关 键 词：虫生真菌 拟青霉菌 杀线虫活性 结构鉴定 

分 类 号：S482.51[农业科学—农药学]