Genome shuffling育种技术应用研究进展  

Researchprogress on the application of Genome shuffling breeding technology

作　　者：孟国庆[1] 陆震[1] 赵林[1] 刘新利[1] 

机构地区：[1]山东轻工业学院山东省微生物工程重点实验室,山东济南250353

出　　处：《山东轻工业学院学报（自然科学版）》2010年第2期6-11,共6页Journal of Shandong Polytechnic University

基　　金：国家科技支撑计划"抗生素战略品种的菌种选育研究"资助项目(No.2007BAI26B01)

摘　　要：本文综述了一种高效育种技术Genome shuffling(基因组重组)技术及其在细菌、真菌及放线菌的菌种选育方面的应用研究进展。与传统育种技术相比,Genome shuffling技术是一种全基因组重排的高效定向育种技术。菌株经过多轮递归原生质体融合,使正向突变的不同基因重组到同一个融合子中,大幅提高菌种的正突变性,打破在菌种改良过程中的诸多局限性,在现代菌种选育中得以广泛应用。In this paper,Genome shuffling as an efficient breeding technology has been widely used in the screen of bacteria,fungi,actinomycetes and so on.Compared with the traditional breeding techniques,Genome shuffling technology is a kind of efficient directly breeding technology through genome rearrangement.The different gene after forward mutant of the strains is recombined to the same fusant through several rounds of protoplast fusion,which can improve the positive mutagenicity of strains and break various limi...

关 键 词：GENOME shuffling技术 菌种选育 原生质体融合 高通量筛选 

分 类 号：Q939.9[生物学—微生物学]