细粒棘球绦虫重组Bb-Eg95-EgA31疫苗免疫小鼠后脾细胞亚群的动态观察  

Dynamic Observation on Splenocyte Subsets in Mice Immunized with Recombinant Bb-Eg95-EgA31 Vaccine of Echinococcus granulosus

作　　者：周必英[1,2] 陈雅棠[1] 李文桂[1] 杨梅[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016 [2]贵州省遵义医学院寄生虫学教研室,遵义563003

出　　处：《四川大学学报（医学版）》2010年第5期764-766,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30801052;30671835;30500423和30200239)资助

摘　　要：目的动态观察细粒棘球绦虫(Eg)重组双歧杆菌(Bb)-Eg95-EgA31疫苗免疫小鼠后脾细胞亚群的变化。方法将5×108克隆形成单位(CFU)和5×105CFU疫苗分别采用口服灌胃和鼻腔内接种免疫BALB/c鼠,在免疫后0、2、4、6、8、10、12、14、16、18和20周各剖杀4只小鼠,取脾,分离脾细胞,用流式细胞术(FCM)检测免疫小鼠脾CD4+和CD8+T细胞亚群的百分比,同时设双歧杆菌液体培养基(MRS)对照。结果口服灌胃组的脾CD4+T细胞亚群在免疫后4~10周升高,免疫后6周达最高水平,CD8+T细胞亚群无明显变化;鼻腔内接种组脾CD4+T细胞亚群在免疫后4~8周升高,免疫后6周达最高水平,CD8+T细胞亚群无明显变化。结论 CD4+T细胞亚群在细粒棘球绦虫重组Bb-Eg95-EgA31疫苗诱导的小鼠免疫应答中起关键作用。Objective To dynamically observe changes of subsets of splenocytes in mice immunized with recombinant bifidobacteria bifidum(Bb)-Eg95-EgA31 vaccine of Echinococcus granulosus(Eg).Methods BALB/c mice were vaccinated by 5×108 colony forming unit(CFU) orally and 5×105 CFU intranasally respectively.Mice were killed on week 0,2,4,6,8,10,12,14,16,18 and 20 after immunization respectively,and spleens were separated for cell preparation.CD4+ and CD8+T cells were determined by flow cytometry(FCM),with MRS as control.Results In the oral immunization group,CD4+ cells showed a significant increase during the 4th-10th week after vaccination,and reached the highest level on the 6th week,whereas no obvious changes in CD8+ cells numbers were observed.In the intranasal immunization group,CD4+ cells showed an obvious increase during the 4th-8th week after vaccination,and reached the highest level on the 6th week,CD8+ subsets had no obvious changes.Conclusion CD4+ T cell cells may play a key role in immune response in mice immunized with the recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus.

关 键 词：细粒棘球绦虫 重组BB-EG95-EGA31疫苗 脾细胞亚群 流式细胞术 

分 类 号：R392[医药卫生—免疫学]