大鼠脑微血管内皮细胞的分离、培养及不同纯化方法的比较  

Separation,Culture and Purification of Rat Brain Microendothelial Cell

作　　者：田林郁[1] 李胜富[2] 周东[1] 

机构地区：[1]四川大学华西医院神经内科,成都610041 [2]四川大学华西医院移植免疫实验室,成都610041

出　　处：《四川大学学报（医学版）》2010年第5期869-872,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号0040205401094)资助

摘　　要：目的对脑微血管内皮细胞分离、培养方法进行探索性研究,并对目前常用的纯化方法进行比较。方法首先采用两次酶消化、两次梯度离心法对大鼠脑血管内皮原代细胞进行分离,然后分别采用免疫磁珠和Thy1.1抗体杀伤法分别进行纯化。结果采用两次酶消化、两次梯度离心法可以成功分离到脑微血管内皮细胞,磁珠纯化法可得到纯度高但是数量较少的细胞,Thy1.1抗体杀伤法可得到纯度及得率均较高的内皮细胞。结论我们所摸索的方法能成功进行纯度较高的大鼠脑微血管内皮细胞原代培养。Objective To develop a separation method for brain microendothelial cells with the comparison of other ones.Methods Twice enzymatic digestion and twice gradient centrifugation were applied to separate rat brain microendothelial cells.Then,immunomagnetic beads and Thy1.1 antibody were used respectively to purify the cultured cells.Results Twice enzymatic digestion and twice gradient centrifugation could separate the cell successfully.High purification but low cell yield was obtained with immunomagnetic beads.The cells handled with Thy1.1 antibody had both higher purify coefficient and higher yield.Conclusion The developed method could separate the brain microendothelial cells successfully.

关 键 词：脑微血管内皮细胞 原代细胞 大鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]