乳化异氟醚灌胃对大鼠的镇静催眠作用与安全性  

The Sedative and Hypnotic Effects and Safety of Oral Emulsified Isoflurane in Rats

作　　者：林海霞[1,2] 罗南富[2] 刘进[2] 杨静[2] 李崎[2] 

机构地区：[1]四川大学华西药学院药理与生物制药系,成都610041 [2]四川大学华西医院麻醉与危重急救研究室,成都610041

出　　处：《四川大学学报（医学版）》2010年第5期862-864,887,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的研究乳化异氟醚对大鼠灌胃给药是否产生镇静催眠作用。方法选择健康SD大鼠,采用序贯法测量大鼠灌胃8%的乳化异氟醚后产生催眠作用的半数有效量(median effective dose,ED50)。求得ED50后,选择健康SD大鼠30只,随机分成3组:乳化异氟醚组(Eiso),灌胃8%乳化异氟醚2.15 mL/100 g〔在ED50基础上增加一个公比r(r=0.8序贯法求催眠作用的剂量公比),即ED50/r=2.15 mL/100 g〕;生理盐水组(NS),灌胃生理盐水2.15 mL/100 g;脂肪乳组(IL),灌胃30%脂肪乳2.15 mL/100 g,每组10只。观察大鼠给药后的神经行为学变化,翻正反射发生率(入睡率),睡眠潜伏期、持续期。结果乳化异氟醚灌胃给药对大鼠有镇静催眠的作用;产生催眠作用的ED50为1.72 mL/100 g;2.15 mL/100 g乳化异氟醚(8%)灌胃后,大鼠翻正反射发生率为90%,睡眠潜伏期约为15~20 min,睡眠持续期长达60 min;给药后观察,大鼠能保持良好的自主呼吸,口唇红润,无腹泻等不良反应。结论乳化异氟醚灌胃能对大鼠产生有效的可逆的镇静催眠作用,并且具有良好的安全性。Objective To investigate the sedative and hypnotic effects and safety of oral emulsified isoflurane in rats.Methods Thirty healthy Sprague-Dawley(SD) rats were randomly divided into three groups.The rats in the emulsified isoflurane group were orally administered with emulsified isoflurane with the sequential method,while the rats in the normal sodium group and the intralipid group were orally administered with sodium and intralipid respectively.The rats in the emulsified isoflurane group received 2.15 mL/100 g of 8% emulsified isoflurane,a dosage calculated with an increase of a common ratio(r=0.8) on the basis of median effective dose(ED50).Similarly,2.15 mL/100 g of normal sodium and 2.15 mL/100 g of 30% intralipid were given to the rats in the normal sodium group and the intralipid group,respectively.The neurobehaviors of the rats were assessed,and the interval of disappearance and recovery of the righting reflex were recorded.Results Oral emulsified isoflurane reduced the autonomic activity time and induced the loss of righting reflex.The ED50 of the loss of righting reflex was 1.72 mL/100 g.The rats orally administered with 2.15 mL/100 g of 8% emulsified isoflurane had 90% of loss of righting reflex,with a sleeping latency of 15-20 min and up to 60 min of sleeping duration.Conclusion Oral emulsified isoflurane has obvious sedative and hypnotic effects.

关 键 词：乳化异氟醚 序贯法 半数有效量 神经行为学 

分 类 号：R96[医药卫生—药理学]