姜黄素对低氧下肺成纤维细胞增殖及Ⅰ型胶原表达的抑制作用研究  

Curcumin Inhibits Hypoxia-induced Proliferation of MRC-5 and Collagen Ⅰ Synthesis

作　　者：何正平[1] 谢敏[1] 

机构地区：[1]四川大学华西医院呼吸内科,成都610041

出　　处：《四川大学学报（医学版）》2010年第4期621-625,共5页Journal of Sichuan University(Medical Sciences)

基　　金：四川省支撑课题(004025301469)资助

摘　　要：目的探讨姜黄素对人胚肺成纤维细胞MRC-5在低氧下增殖和胶原表达中的作用及其机制。方法以人胚肺成纤维细胞MRC-5进行培养,随机分为常氧组(12 h,24 h,36 h)、低氧组(12 h,24 h,36 h)、低氧(12 h,24 h,36 h)+姜黄素(5μmol/L,10μmol/L,20μmol/L)组、低氧(36 h)+p38丝裂原激活蛋白激酶(p38 MAPK)阻断剂SB203580(10μmol/L)组和低氧(36 h)+三磷酸肌醇激酶/蛋白激酶B(PI3K/AKT)阻断剂LY-294002(10μmol/L)组。分别采用MTT法检测细胞增殖情况,Western blot检测MRC-5细胞中磷酸化AKT(p-AKT)、磷酸化P38(p-p38)和Ⅰ型胶原α1前体(pro-col1α1)蛋白的表达情况。结果姜黄素能抑制低氧下肺成纤维细胞的增殖,并且以低氧(36 h)+姜黄素20μmol/L组最为明显,SB203580和LY-294002能抑制MRC-5细胞增殖(P<0.05)。低氧下pro-col1α1、p-AKT、p-p38蛋白表达较常氧下增强(P<0.05),但姜黄素只对低氧下pro-col1α1、p-p38蛋白的表达有抑制,且呈剂量依赖性(P<0.05),姜黄素对p-AKT表达无明显影响(P>0.05)。结论低氧促进MRC-5细胞增殖及胶原表达可能是通过PI3K/AKT及p38 MAPK途径,姜黄素对低氧下MRC-5细胞增殖及I型胶原表达的抑制则可能是通过p38 MAPK实现。Objective To investigate the effect of curcumin on MRC-5 in human embryo fibroblast(HEF) under hypoxia and possible underlying mechanism.Methods The MRC-5 were cultured in vivo under 5 different conditions,including normal control,SB203580(10 μmol/L),LY-294002(10 μmol/L) and curcumin(5,10 and 20 μmol/L,respectively).The proliferation of MRC-5 was assessed by MTT assay and the expressions of pro-col1α1,p-p38 and p-AKT were detected by western blot.Results Compared with the nomoxia control,hypoxia enhanced the proliferation of MRC-5 and the expressions of pro-col1α1,p-p38 and p-AKT(P0.05).Conclusion Hypoxia might enhance the proliferation of MRC-5 cells and the expression of collagen Ⅰ through PI3K/AKT and p38MAPK.Curcumin blocks the hypoxia influence through is impact on p38MAPK.

关 键 词：低氧 人胚肺成纤维细胞 姜黄素 

分 类 号：R285[医药卫生—中药学]