甘草酸对变异链球菌生长和产酸影响的体外研究  

Effects of Glycyrrhizic Acid on the Growth and Acid-producing of Streptococcus mutans in vitro

作　　者：刘果[1] 何永红[1] 张菲菲[2] 孔祥丽[1] 温艳丽[2] 马琴睿[2] 杨英明[2] 万呼春[2] 

机构地区：[1]四川大学口腔疾病研究国家重点实验室,成都610041 [2]四川大学华西口腔医院内科,成都610041

出　　处：《四川大学学报（医学版）》2010年第4期634-637,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的研究甘草酸对体外培养的变异链球菌的抗菌活性。方法采用液体稀释法测定甘草酸对变异链球菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。在不同浓度甘草酸药液中培养变异链球菌,分别于培养0 h、3 h、7 h、12 h、23 h和40 h时测定细菌悬液吸光度值和培养液pH值,绘制变异链球菌的生长曲线和产酸曲线,计算生长抑制率和产酸抑制率。结果甘草酸对变异链球菌的MIC为1.57 mg/mL,浓度≤12.5 mg/mL的甘草酸无杀菌作用。甘草酸对变异链球菌生长和产酸均有抑制作用,作用随甘草酸浓度的增加而增强。当浓度≥1.57mg/mL时,甘草酸对变异链球菌生长和产酸抑制率分别在80%和70%以上。结论甘草酸对体外培养变异链球菌的生长和产酸均有抑制作用。Objective To investigate the antibacterial activity of glycyrrhizic acid against Streptococcus mutans(S.mutans,ATCC 25175) in vitro.Methods The minimal inhibitory concentration(MIC) and the minimal bactericidal concentration(MBC) of glycyrrhizic acid against S.mutans were detected using doubling dilution.The effects of glycyrrhizic acid on the growth and acidogenic profile of S.mutans and the inhibition ratio of glycyrrhizic acid on growth and acid-producing of S.mutans were investigated by detecting the Abs of bacteria suspension and the pH value of medium at definite time intervals(0 h,3 h,7 h,12 h,23 h,40 h) during cultivation.Results The MIC determined for glycyrrhizic acid was 1.57 mg/mL and there was no bactericidal effect when concentration of glycyrrhizic acid up to 12.5 mg/mL.The glycyrrhizic acid inhibitted the multiplication and acid-producing of S.mutans significantly and the effects became stronger with concentration increasing.When concentration up to 1.57 mg/mL,the inhibition ratio of glycyrrhizic acid on the growth and acid-producing of S.mutans were exceeded 80 and 70 percent,respectively.Conclusion The glycyrrhizic acid can inhibit the growth and acid-producing of S.mutans in vitro.

关 键 词：甘草酸 变异链球菌 生长抑制 产酸抑制 

分 类 号：R285.5[医药卫生—中药学]